ArticleGene therapy2007
Gene silencing by the tRNA maturase tRNase ZL under the direction of small-guide RNA.
Article in Gene therapy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- TRUE Gene Silencing.International journal of molecular sciences · 2022Review
- The heptamer sgRNA targeting the human OCT4 mRNA can upregulate the OCT4 expression.Biochemistry and biophysics reports · 2021Article
- The computational approaches of lncRNA identification based on coding potential:Computational and structural biotechnology journal · 2020Review
- Potential physiological roles of the 31/32-nucleotide Y4-RNA fragment in human plasma.Non-coding RNA research · 2019Article
- Minimal requirements for reverse polymerization and tRNA repair by tRNARNA biology · 2018Article
- The Y4-RNA fragment, a potential diagnostic marker, exists in saliva.Non-coding RNA research · 2017Article
- TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents.Journal of visualized experiments : JoVE · 2016Article
- Potential small guide RNAs for tRNase ZL from human plasma, peripheral blood mononuclear cells, and cultured cell lines.PloS one · 2015Article
- Characterization of the small RNA content of Trypanosoma cruzi extracellular vesicles.Molecular and biochemical parasitology · 2014Article
- Extracellular vesicles shed by Trypanosoma cruzi are linked to small RNA pathways, life cycle regulation, and susceptibility to infection of mammalian cells.Parasitology research · 2014Article
- Growth inhibition of head and neck squamous cell carcinoma cells by sgRNA targeting the cyclin D1 mRNA based on TRUE gene silencing.PloS one · 2014Article
- Developmental roles of Drosophila tRNA processing endonuclease RNase ZL as revealed with a conditional rescue system.Developmental biology · 2013Article
- Article
- Elimination of specific miRNAs by naked 14-nt sgRNAs.PloS one · 2012Article
- Identification of microRNA precursors based on random forest with network-level representation method of stem-loop structure.BMC bioinformatics · 2011Article
- Short RNA duplexes guide sequence-dependent cleavage by human Dicer.RNA (New York, N.Y.) · 2010Article
- Article
- Identification and classification of ncRNA molecules using graph properties.Nucleic acids research · 2009Article
- Optimization and characterization of tRNA-shRNA expression constructs.Nucleic acids research · 2007Article
- 3' processing of eukaryotic precursor tRNAs.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have been developing a unique system for the downregulation of a gene expression through cutting a specific mRNA by the long form of tRNA 3'-processing endoribonuclease (tRNase Z(L)) under the direction of small-guide RNA (sgRNA). However, the efficacy of this system and the involvement of tRNase Z(L) in the living cells were not clear. Here we show, by targeting the exogenous luciferase gene, that the efficacy of the sgRNA/tRNase Z(L) method can become comparable to that of the RNA interference technology and that the gene silencing is owing to tRNase Z(L) directed by sgRNA not owing to a simple antisense effect. We also show that tRNase Z(L) together with sgRNA can downregulate expression of the endogenous human genes Bcl-2 and glycogen synthase kinase-3beta by degrading their mRNAs in cell culture. Furthermore, we demonstrate that a gene expression in the livers of postnatal mice can be inhibited by an only seven-nucleotide sgRNA. These data suggest that sgRNA might be utilized as therapeutic agents to treat diseases such as cancers and AIDS.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.