ArticleJournal of bacteriology2006
Interaction of TonB with the outer membrane receptor FpvA of Pseudomonas aeruginosa.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- Structural constraints of pyocin S2 import through the ferripyoverdine receptor FpvAI.PNAS nexus · 2024Article
- Mobilization of Iron Stored in Bacterioferritin Is Required for Metabolic Homeostasis inPathogens (Basel, Switzerland) · 2020Article
- Article
- Time-Course Proteomic Analysis ofPolymers · 2019Article
- Genome-Based Metabolic Reconstruction Unravels the Key Role of B12 in Methionine Auxotrophy of anFrontiers in microbiology · 2019Article
- The biosynthesis of pyoverdines.Microbial cell (Graz, Austria) · 2018Review
- Fermentation products in the cystic fibrosis airways induce aggregation and dormancy-associated expression profiles in a CF clinical isolate of Pseudomonas aeruginosa.FEMS microbiology letters · 2018Article
- Exploitation of an iron transporter for bacterial protein antibiotic import.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Design, synthesis and microbiological evaluation of ampicillin-tetramic acid hybrid antibiotics.The Journal of antibiotics · 2017Article
- Monomeric TonB and the Ton box are required for the formation of a high-affinity transporter-TonB complex.Biochemistry · 2013Article
- Deciphering protein dynamics of the siderophore pyoverdine pathway in Pseudomonas aeruginosa.PloS one · 2013Article
- Role of TonB1 in pyoverdine-mediated signaling in Pseudomonas aeruginosa.Journal of bacteriology · 2009Article
- The Pseudomonas aeruginosa pyochelin-iron uptake pathway and its metal specificity.Journal of bacteriology · 2009Article
- Signaling mechanisms for activation of extracytoplasmic function (ECF) sigma factors.Biochimica et biophysica acta · 2008Review
- Passively released heme from hemoglobin and myoglobin is a potential source of nutrient iron for Bordetella bronchiseptica.Infection and immunity · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyoverdine-mediated iron uptake by the FpvA receptor in the outer membrane of Pseudomonas aeruginosa is dependent on the inner membrane protein TonB1. This energy transducer couples the proton-electrochemical potential of the inner membrane to the transport event. To shed more light upon this process, a recombinant TonB1 protein lacking the N-terminal inner membrane anchor (TonB(pp)) was constructed. This protein was, after expression in Escherichia coli, purified from the soluble fraction of lysed cells by means of an N-terminal hexahistidine or glutathione S-transferase (GST) tag. Purified GST-TonB(pp) was able to capture detergent-solubilized FpvA, regardless of the presence of pyoverdine or pyoverdine-Fe. Targeting of the TonB1 fragment to the periplasm of P. aeruginosa inhibited the transport of ferric pyoverdine by FpvA in vivo, indicating an interference with endogenous TonB1, presumably caused by competition for binding sites at the transporter or by formation of nonfunctional TonB heterodimers. Surface plasmon resonance experiments demonstrated that the FpvA-TonB(pp) interactions have apparent affinities in the micromolar range. The binding of pyoverdine or ferric pyoverdine to FpvA did not modulate this affinity. Apparently, the presence of either iron or pyoverdine is not essential for the formation of the FpvA-TonB complex in vitro.
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