ArticleApplied and environmental microbiology2006
Random insertion of a TetR-inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- A multi-tetracycline responsive induction system for gene expression inApplied and environmental microbiology · 2026Article
- A Multi-Tetracycline Responsive Induction System for Gene Expression inbioRxiv : the preprint server for biology · 2025Article
- Status quo of tet regulation in bacteria.Microbial biotechnology · 2022Review
- Promoter strength driving TetR determines the regulatory properties of Tet-controlled expression systems.PloS one · 2012Article
- Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag-induced reporter fluorescence.Microbial biotechnology · 2012Article
- Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control.PloS one · 2008Article
- The application of Tet repressor in prokaryotic gene regulation and expression.Microbial biotechnology · 2008Review
- Efficient and exclusive induction of Tet repressor by the oligopeptide Tip results from co-variation of their interaction site.Nucleic acids research · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The insertion element InsTipalpha was constructed to generate protein expression data. It randomly fuses the TetR-inducing peptide Tip to the affected reading frame. Fusion protein expression is quantified by Tet-regulated reporter gene expression. The expression patterns of tagged Escherichia coli genes fully agree with published data from transcriptional fusions or microarrays, validating the Tip tag approach.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.