ArticleApplied and environmental microbiology2006
Improvement of lactic acid production in Saccharomyces cerevisiae by cell sorting for high intracellular pH.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 122 citations in OpenAlex.
- Understanding the Role of pH Regulation and Neutralizing Agents in Organic Acid Production and Growth of Aspergillus oryzae.Biotechnology and bioengineering · 2026Article
- Long-term adaptive laboratory evolution of Saccharomyces cerevisiae for high-titer lactic acid production at low pH.Microbial cell factories · 2025Article
- Metabolic engineering and adaptive laboratory evolution of Kluyveromyces Marxianus for lactic acid production.Microbial cell factories · 2025Article
- Valorisation of cotton post-industrial textile waste into lactic acid: chemo-mechanical pretreatment, separate hydrolysis and fermentation using engineered yeast.Microbial cell factories · 2024Article
- Just around the Corner: Advances in the Optimization of Yeasts and Filamentous Fungi for Lactic Acid Production.Journal of fungi (Basel, Switzerland) · 2024Review
- Metabolic Engineering and Adaptive Evolution for Efficient Production of l-Lactic Acid in Saccharomyces cerevisiae.Microbiology spectrum · 2022Article
- Autotrophic lactate production from HApplied microbiology and biotechnology · 2022Article
- Mechanisms underlying lactic acid tolerance and its influence on lactic acid production inMicrobial cell (Graz, Austria) · 2021Review
- Lactic acid production - producing microorganisms and substrates sources-state of art.Heliyon · 2020Review
- Downscaling screening cultures in a multifunctional bioreactor array-on-a-chip for speeding up optimization of yeast-based lactic acid bioproduction.Biotechnology and bioengineering · 2020Article
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- Weak Acid Permeation in Synthetic Lipid Vesicles and Across the Yeast Plasma Membrane.Biophysical journal · 2020Article
- Activation of Haa1 and War1 transcription factors by differential binding of weak acid anions in Saccharomyces cerevisiae.Nucleic acids research · 2019Article
- Evolutionary engineering inBiotechnology for biofuels · 2019Article
- Electrooptical Determination of Polarizability for On-Line Viability and Vitality Quantification ofFrontiers in bioengineering and biotechnology · 2018Article
- Metabolic engineering ofMetabolic engineering communications · 2017Article
- Beyond the bulk: disclosing the life of single microbial cells.FEMS microbiology reviews · 2017Review
- Engineering a riboswitch-based genetic platform for the self-directed evolution of acid-tolerant phenotypes.Nature communications · 2017Article
- Technological Microbiology: Development and Applications.Frontiers in microbiology · 2017Review
- GSF2 deletion increases lactic acid production by alleviating glucose repression in Saccharomyces cerevisiae.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yeast strains expressing heterologous L-lactate dehydrogenases can produce lactic acid. Although these microorganisms are tolerant of acidic environments, it is known that at low pH, lactic acid exerts a high level of stress on the cells. In the present study we analyzed intracellular pH (pHi) and viability by staining with cSNARF-4F and ethidium bromide, respectively, of two lactic-acid-producing strains of Saccharomyces cerevisiae, CEN.PK m850 and CEN.PK RWB876. The results showed that the strain producing more lactic acid, CEN.PK m850, has a higher pHi. During batch culture, we observed in both strains a reduction of the mean pHi and the appearance of a subpopulation of cells with low pHi. Simultaneous analysis of pHi and viability proved that the cells with low pHi were dead. Based on the observation that the better lactic-acid-producing strain had a higher pHi and that the cells with low pHi were dead, we hypothesized that we might find better lactic acid producers by screening for cells within the highest pHi range. The screening was performed on UV-mutagenized populations through three consecutive rounds of cell sorting in which only the viable cells within the highest pHi range were selected. The results showed that lactic acid production was significantly improved in the majority of the mutants obtained compared to the parental strains. The best lactic-acid-producing strain was identified within the screening of CEN.PK m850 mutants.
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