ArticleApplied and environmental microbiology2006
Targeting species-specific low-affinity 16S rRNA binding sites by using peptide nucleic acids for detection of Legionellae in biofilms.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- Article
- Legionella colonization and 3D spatial location within a Pseudomonas biofilm.Scientific reports · 2024Article
- Ecology ofBiofilm · 2024Review
- Development of a Novel Peptide Nucleic Acid Probe for the Detection ofMicroorganisms · 2022Article
- Antisense peptide nucleic acids as a potential anti-infective agent.Journal of microbiology (Seoul, Korea) · 2019Review
- DNA nanotechnology for nucleic acid analysis: multifunctional molecular DNA machine for RNA detection.Chemical communications (Cambridge, England) · 2016Article
- Determination of viable legionellae in engineered water systems: Do we find what we are looking for?Water research · 2016Review
- Detection of bacterial 16S rRNA using a molecular beacon-based X sensor.Biosensors & bioelectronics · 2013Article
- Molecular-beacon-based tricomponent probe for SNP analysis in folded nucleic acids.Chemistry (Weinheim an der Bergstrasse, Germany) · 2011Article
- Interaction of Legionella pneumophila and Helicobacter pylori with bacterial species isolated from drinking water biofilms.BMC microbiology · 2011Article
- Two Legionnaires' disease cases associated with industrial waste water treatment plants: a case report.BMC infectious diseases · 2010Article
- Fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of Salmonella spp. in a broad spectrum of samples.Applied and environmental microbiology · 2010Article
- Persistence of Helicobacter pylori in heterotrophic drinking-water biofilms.Applied and environmental microbiology · 2008Article
- DNA mimics for the rapid identification of microorganisms by fluorescence in situ hybridization (FISH).International journal of molecular sciences · 2008Article
- Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsy specimens.Journal of clinical microbiology · 2007Article
- Survival of Mycobacterium avium, Legionella pneumophila, Escherichia coli, and caliciviruses in drinking water-associated biofilms grown under high-shear turbulent flow.Applied and environmental microbiology · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using fluorescence in situ hybridization to detect bacterial groups has several inherent limitations. DNA probes are generally used, targeting sites on the 16S rRNA. However, much of the 16S rRNA is highly conserved, with variable regions often located in inaccessible areas where secondary structures can restrict probe access. Here, we describe the use of peptide nucleic acid (PNA) probes as a superior alternative to DNA probes, especially when used for environmental samples. A complex bacterial genus (Legionella) was studied, and two probes were designed, one to detect all species and one targeted to Legionella pneumophila. These probes were developed from existing sequences and are targeted to low-binding-affinity sites on the 16S rRNA. In total, 47 strains of Legionella were tested. In all cases, the Legionella spp. PNA probe labeled cells strongly but did not bind to any non-Legionella species. Likewise, the specific L. pneumophila PNA probe labeled only strains of L. pneumophila. By contrast, the equivalent DNA probes performed poorly. To assess the applicability of this method for use on environmental samples, drinking-water biofilms were spiked with a known concentration of L. pneumophila bacteria. Quantifications of the L. pneumophila bacteria were compared using PNA hybridization and standard culture methods. The culture method quantified only 10% of the number of L. pneumophila bacteria found by PNA hybridization. This illustrates the value of this method for use on complex environmental samples, especially where cells may be in a viable but noncultivable state.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.