ArticleApplied and environmental microbiology2006
Real-time detection of noroviruses in surface water by use of a broadly reactive nucleic acid sequence-based amplification assay.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 81 citations in OpenAlex.
- Enhanced Specificity in Colorimetric LAMP Assay forJournal of fungi (Basel, Switzerland) · 2024Article
- Molecular and Genetics-Based Systems for Tracing the Evolution and Exploring the Mechanisms of Human Norovirus Infections.International journal of molecular sciences · 2023Review
- Prevalence, Distribution, and Genotypes of Adenovirus and Norovirus in the Puzi River and Its Tributaries and the Surrounding Areas in Taiwan.GeoHealth · 2021Article
- Review on the contamination of wastewater by COVID-19 virus: Impact and treatment.The Science of the total environment · 2021Review
- Critical review of methods for isothermal amplification of nucleic acids for environmental analysis.Journal of microbiological methods · 2020Review
- Review
- Low-cost detection of norovirus using paper-based cell-free systems and synbody-based viral enrichment.Synthetic biology (Oxford, England) · 2018Article
- Washed Away; How Not to Lose Your RNA during Isolation.Journal of biomolecular techniques : JBT · 2017Article
- Bayesian Modeling of Enteric Virus Density in Wastewater Using Left-Censored Data.Food and environmental virology · 2013Article
- Application of a receptor-binding capture quantitative reverse transcription-PCR assay to concentrate human norovirus from sewage and to study the distribution and stability of the virus.Applied and environmental microbiology · 2012Article
- Concentration and diversity of noroviruses detected in Luxembourg wastewaters in 2008-2009.Applied and environmental microbiology · 2011Article
- Meeting report: knowledge and gaps in developing microbial criteria for inland recreational waters.Environmental health perspectives · 2010Article
- New tools for the study and direct surveillance of viral pathogens in water.Current opinion in biotechnology · 2008Review
- Evaluation of the persistence of infectious human noroviruses on food surfaces by using real-time nucleic acid sequence-based amplification.Applied and environmental microbiology · 2008Article
- Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure.Applied and environmental microbiology · 2008Article
- Gastroenteritis outbreak caused by waterborne norovirus at a New Zealand ski resort.Applied and environmental microbiology · 2007Article
- Molecular identification and genetic analysis of Norovirus genogroups I and II in water environments: comparative analysis of different reverse transcription-PCR assays.Applied and environmental microbiology · 2007Article
- Chapter 9 The Detection of Waterborne Viruses.Perspectives in medical virology · 2007Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Noroviruses are the most common agents causing outbreaks of viral gastroenteritis. Outbreaks originating from contaminated drinking water and from recreational waters have been described. Due to a lack of cell culture systems, noroviruses are detected mostly by molecular methods. Molecular detection assays for viruses in water are often repressed by inhibitory factors present in the environment, like humic acids and heavy metals. To study the effect of environmental inhibitors on the performance of nucleic acid sequence-based amplification (NASBA), we developed a real-time norovirus NASBA targeting part of the RNA-dependent RNA polymerase (RdRp) gene. Specificity of the assay was studied with 33 divergent clones that contained part of the targeted RdRp gene of noroviruses from 15 different genogroups. Viral RNA originated from commercial oysters, surface waters, and sewage treatment plants in The Netherlands. Ninety-seven percent of the clones derived from human noroviruses were detected by real-time NASBA. Two clones containing animal noroviruses were not detected by NASBA. We compared the norovirus detection by real-time NASBA with that by conventional reverse transcriptase PCR (RT-PCR) with large-volume river water samples and found that inhibitory factors of RT-PCR had little or no effect on the performance of the norovirus NASBA. This consequently resulted in a higher sensitivity of the NASBA assay than of the RT-PCR. We show that by combining an efficient RNA extraction method with real-time NASBA the sensitivity of norovirus detection in water samples increased at least 100 times, which consequently has implications for the outcome of the infectious risk assessment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.