ArticleApplied and environmental microbiology2006
Improved in situ hybridization efficiency with locked-nucleic-acid-incorporated DNA probes.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 98 citations in OpenAlex.
- Deep-tissue transcriptomics and subcellular imaging at high spatial resolution.Science (New York, N.Y.) · 2025Article
- High throughput AS LNA qPCR method for the detection of a specific mutation in poliovirus vaccine strains.Vaccine · 2024Article
- An Advanced Detection System forActa histochemica et cytochemica · 2022Article
- Bioinformatic Tools and Guidelines for the Design of Fluorescence In Situ Hybridization Probes.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Epigenetic Landscape of Liquid Biopsy in Colorectal Cancer.Frontiers in cell and developmental biology · 2021Review
- Endodontic-Like Oral Biofilms as Models for Multispecies Interactions in Endodontic Diseases.Microorganisms · 2020Article
- Optimizing locked nucleic acid/2'-O-methyl-RNA fluorescence in situ hybridization (LNA/2'OMe-FISH) procedure for bacterial detection.PloS one · 2019Article
- Phosphorothioate-Modified AP613-1 Specifically Targets GPC3 when Used for Hepatocellular Carcinoma Cell Imaging.Molecular therapy. Nucleic acids · 2018Article
- Unlocked nucleic acid modified primer-based enzymatic polymerization assay: towards allele-specific genotype detection of human platelet antigens.RSC advances · 2018Article
- Evaluation of fluorescence in situ hybridization techniques to study long non-coding RNA expression in cultured cells.Nucleic acids research · 2018Article
- Phylogenetic diversity and in situ detection of eukaryotes in anaerobic sludge digesters.PloS one · 2017Article
- DNA nanotechnology for nucleic acid analysis: multifunctional molecular DNA machine for RNA detection.Chemical communications (Cambridge, England) · 2016Article
- Fluorescence based fiber optic and planar waveguide biosensors. A review.Analytica chimica acta · 2016Review
- Design a cadA-targeted DNA probe for screening of potential bacterial cadmium biosorbents.Environmental science and pollution research international · 2016Article
- Heterogeneity of miR-10b expression in circulating tumor cells.Scientific reports · 2015Article
- Detection of the myxosporean parasite Parvicapsula pseudobranchicola in Atlantic salmon (Salmo salar L.) using in situ hybridization (ISH).Parasites & vectors · 2015Article
- Article
- CARD-FISH for environmental microorganisms: technical advancement and future applications.Microbes and environments · 2013Review
- Hybridization-based detection of Helicobacter pylori at human body temperature using advanced locked nucleic acid (LNA) probes.PloS one · 2013Article
- Demonstration of hepatitis C virus RNA with in situ hybridization employing a locked nucleic Acid probe in humanized liver of infected chimeric mice and in needle-biopsied human liver.International journal of hepatology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Low signal intensity due to poor probe hybridization efficiency is one of the major drawbacks of rRNA-targeted in situ hybridization. There are two major factors affecting the hybridization efficiency: probe accessibility and affinity to the targeted rRNA molecules. In this study, we demonstrate remarkable improvement in in situ hybridization efficiency by applying locked-nucleic-acid (LNA)-incorporated oligodeoxynucleotide probes (LNA/DNA probes) without compromising specificity. Fluorescently labeled LNA/DNA probes with two to four LNA substitutions exhibited strong fluorescence intensities equal to or greater than that of probe Eub338, although these probes did not show bright signals when they were synthesized as DNA probes; for example, the fluorescence intensity of probe Eco468 increased by 22-fold after three LNA bases were substituted for DNA bases. Dissociation profiles of the probes revealed that the dissociation temperature was directly related to the number of LNA substitutions and the fluorescence intensity. These results suggest that the introduction of LNA residues in DNA probes will be a useful approach for effectively enhancing probe hybridization efficiency.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.