ArticleApplied and environmental microbiology2006
The Bacillus subtilis nrdEF genes, encoding a class Ib ribonucleotide reductase, are essential for aerobic and anaerobic growth.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Analysis of essential genes inJournal of bacteriology · 2025Article
- Analysis of Essential Genes inbioRxiv : the preprint server for biology · 2025Article
- Article
- Effects ofAntibiotics (Basel, Switzerland) · 2021Article
- Hydroxyurea Induces a Stress Response That Alters DNA Replication and Nucleotide Metabolism in Bacillus subtilis.Journal of bacteriology · 2021Article
- Role of Ribonucleotide Reductase in Bacillus subtilis Stress-Associated Mutagenesis.Journal of bacteriology · 2017Article
- The Blueprint of a Minimal Cell: MiniBacillus.Microbiology and molecular biology reviews : MMBR · 2016Review
- Global Transcriptional Analysis of Virus-Host Interactions between Phage ϕ29 and Bacillus subtilis.Journal of virology · 2016Article
- De Novo Guanine Biosynthesis but Not the Riboswitch-Regulated Purine Salvage Pathway Is Required for Staphylococcus aureus Infection In Vivo.Journal of bacteriology · 2016Article
- Bacillus subtilis class Ib ribonucleotide reductase: high activity and dynamic subunit interactions.Biochemistry · 2014Article
- SP10 infectivity is aborted after bacteriophage SP10 infection induces nonA transcription on the prophage SPβ region of the Bacillus subtilis genome.Journal of bacteriology · 2014Article
- NrdH-redoxin protein mediates high enzyme activity in manganese-reconstituted ribonucleotide reductase from Bacillus anthracis.The Journal of biological chemistry · 2011Article
- Bacillus subtilis class Ib ribonucleotide reductase is a dimanganese(III)-tyrosyl radical enzyme.Biochemistry · 2011Article
- Implications of the inability of Listeria monocytogenes EGD-e to grow anaerobically due to a deletion in the class III NrdD ribonucleotide reductase for its use as a model laboratory strain.Journal of bacteriology · 2011Article
- COMODO: an adaptive coclustering strategy to identify conserved coexpression modules between organisms.Nucleic acids research · 2011Article
- Class I ribonucleotide reductases: metallocofactor assembly and repair in vitro and in vivo.Annual review of biochemistry · 2011Review
- Diamide triggers mainly S Thiolations in the cytoplasmic proteomes of Bacillus subtilis and Staphylococcus aureus.Journal of bacteriology · 2009Article
- PerR confers phagocytic killing resistance and allows pharyngeal colonization by group A Streptococcus.PLoS pathogens · 2008Article
- Extracytoplasmic processes impaired by inactivation of trxA (thioredoxin gene) in Bacillus subtilis.Journal of bacteriology · 2008Article
- NrdI essentiality for class Ib ribonucleotide reduction in Streptococcus pyogenes.Journal of bacteriology · 2008Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Ribonucleotide reductases (RNRs) are essential for the biosynthesis of the deoxyribonucleoside triphosphates of DNA. Recently, it was proposed that externally supplied deoxyribonucleosides or DNA is required for the growth of Bacillus subtilis under strict anaerobic conditions (M. J. Folmsbee, M. J. McInerney, and D. P. Nagle, Appl. Environ. Microbiol. 70:5252-5257, 2004). Cultivation of B. subtilis on minimal medium in the presence of oxygen indicators in combination with oxygen electrode measurements and viable cell counting demonstrated that growth occurred under strict anaerobic conditions in the absence of externally supplied deoxyribonucleosides. The nrdEF genes encode the only obvious RNR in B. subtilis. A temperature-sensitive nrdE mutant failed to grow under aerobic and anaerobic conditions, indicating that this oxygen-dependent class I RNR has an essential role under both growth conditions. Aerobic growth and anaerobic growth of the nrdE mutant were rescued by addition of deoxynucleotides. The nrd locus consists of an nrdI-nrdE-nrdF-ymaB operon. The 5' end of the corresponding mRNA revealed transcriptional start sites 45 and 48 bp upstream of the translational start of nrdI. Anaerobic transcription of the operon was found to be dependent on the presence of intact genes for the ResDE two-component redox regulatory system. Two potential ResD binding sites were identified approximately 62 bp (site A) and 50 bp (site B) upstream of the transcriptional start sites by a bioinformatic approach. Only mutation of site B eliminated nrd expression. Aerobic transcription was ResDE independent but required additional promoter elements localized between 88 and 275 bp upstream of the transcriptional start.
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