ArticleRespiratory research2006
Laminin-332 alters connexin profile, dye coupling and intercellular Ca2+ waves in ciliated tracheal epithelial cells.
Article in Respiratory research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Retinal capillary basement membrane thickening: Role in the pathogenesis of diabetic retinopathy.Progress in retinal and eye research · 2021Review
- Concatenation of Human Connexin26 (hCx26) and Human Connexin46 (hCx46) for the Analysis of Heteromeric Gap Junction Hemichannels and Heterotypic Gap Junction Channels.International journal of molecular sciences · 2018Article
- Lipidated connexin mimetic peptides potently inhibit gap junction-mediated CaAmerican journal of physiology. Cell physiology · 2018Article
- An autocrine ATP release mechanism regulates basal ciliary activity in airway epithelium.The Journal of physiology · 2017Article
- A transiently expressed connexin is essential for anterior neural plate development in Ciona intestinalis.Development (Cambridge, England) · 2013Article
- Laminin-111 stimulates proliferation of mouse embryonic stem cells through a reduction of gap junctional intercellular communication via RhoA-mediated Cx43 phosphorylation and dissociation of Cx43/ZO-1/drebrin complex.Stem cells and development · 2012Article
- Laminin α1 is essential for mouse cerebellar development.Matrix biology : journal of the International Society for Matrix Biology · 2012Article
- The extracellular signal-regulated kinase mitogen-activated protein kinase/ribosomal S6 protein kinase 1 cascade phosphorylates cAMP response element-binding protein to induce MUC5B gene expression via D-prostanoid receptor signaling.The Journal of biological chemistry · 2011Article
- Spontaneous lung dysfunction and fibrosis in mice lacking connexin 40 and endothelial cell connexin 43.The American journal of pathology · 2011Article
- Potent agonists of the protease activated receptor 2 (PAR2).Journal of medicinal chemistry · 2011Article
- Ca2+ signaling in airway epithelial cells facilitates leukocyte recruitment and transepithelial migration.Journal of leukocyte biology · 2009Review
- The extracellular matrix controls gap junction protein expression and function in postnatal hippocampal neural progenitor cells.BMC neuroscience · 2009Article
- TLR2 regulates gap junction intercellular communication in airway cells.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundTracheal epithelial cells are anchored to a dynamic basement membrane that contains a variety of extracellular matrix proteins including collagens and laminins. During development, wound repair and disease of the airway epithelium, significant changes in extracellular matrix proteins may directly affect cell migration, differentiation and events mediated by intercellular communication. We hypothesized that alterations in cell matrix, specifically type I collagen and laminin alpha3beta3gamma2 (LM-332) proteins within the matrix, directly affect intercellular communication in ciliated rabbit tracheal epithelial cells (RTEC).
methodsFunctional coupling of RTEC was monitored by microinjection of the negatively charged fluorescent dyes, Lucifer Yellow and Alexa 350, into ciliated RTEC grown on either a LM-332/collagen or collagen matrix. Coupling of physiologically significant molecules was evaluated by the mechanism and extent of propagated intercellular Ca2+ waves. Expression of connexin (Cx) mRNA and proteins were assayed by reverse transcriptase - polymerase chain reaction and immunocytochemistry, respectively.
resultsWhen compared to RTEC grown on collagen alone, RTEC grown on LM-332/collagen displayed a significant increase in dye transfer. Although mechanical stimulation of RTEC grown on either LM-332/collagen or collagen alone resulted in intercellular Ca2+ waves, the mechanism of transfer was dependent on matrix: RTEC grown on LM-332/collagen propagated Ca2+waves via extracellular purinergic signaling whereas RTEC grown on collagen used gap junctions. Comparison of RTEC grown on collagen or LM-332/collagen matrices revealed a reorganization of Cx26, Cx43 and Cx46 proteins.
conclusionAlterations in airway basement membrane proteins such as LM-332 can induce connexin reorganizations and result in altered cellular communication mechanisms that could contribute to airway tissue function.
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