ArticleGenes & development2006
In vivo commitment to yeast cotranscriptional splicing is sensitive to transcription elongation mutants.
Article in Genes & development, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 80 citations in OpenAlex.
- Emerging and re-emerging themes in co-transcriptional pre-mRNA splicing.Molecular cell · 2024Review
- Co-transcriptional gene regulation in eukaryotes and prokaryotes.Nature reviews. Molecular cell biology · 2024Review
- Recurrent SRSF2 mutations in MDS affect both splicing and NMD.Genes & development · 2020Article
- Nascent RNA and the Coordination of Splicing with Transcription.Cold Spring Harbor perspectives in biology · 2019Review
- Emerging roles of histone modifications and HDACs in RNA splicing.Nucleic acids research · 2019Review
- Pre-mRNA Splicing in the Nuclear Landscape.Cold Spring Harbor symposia on quantitative biology · 2019Article
- Mud2 functions in transcription by recruiting the Prp19 and TREX complexes to transcribed genes.Nucleic acids research · 2018Article
- Article
- The histone variant H2A.Z promotes efficient cotranscriptional splicing inGenes & development · 2017Article
- Perfect timing: splicing and transcription rates in living cells.Wiley interdisciplinary reviews. RNA · 2017Review
- Subgenic Pol II interactomes identify region-specific transcription elongation regulators.Molecular systems biology · 2017Article
- Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue.Cell reports · 2016Article
- Article
- Histone H3K36 methylation regulates pre-mRNA splicing in Saccharomyces cerevisiae.RNA biology · 2016Article
- Chemistry and Biology of Self-Cleaving Ribozymes.Trends in biochemical sciences · 2015Review
- Mechanisms and Regulation of Alternative Pre-mRNA Splicing.Annual review of biochemistry · 2015Review
- Design and application of cotranscriptional non-enzymatic RNA circuits and signal transducers.Nucleic acids research · 2014Article
- An unanticipated early function of DEAD-box ATPase Prp28 during commitment to splicing is modulated by U5 snRNP protein Prp8.RNA (New York, N.Y.) · 2014Article
- Adventures in time and space: splicing efficiency and RNA polymerase II elongation rate.RNA biology · 2014Review
- Cotranscriptional recruitment of yeast TRAMP complex to intronic sequences promotes optimal pre-mRNA splicing.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Spliceosome assembly in the budding yeast Saccharomyces cerevisiae was recently shown to occur at the site of transcription. However, evidence for cotranscriptional splicing as well as for coupling between transcription and splicing is still lacking. Using modifications of a previously published chromatin immunoprecipitation (ChIP) assay, we show that cotranscriptional splicing occurs approximately 1 kb after transcription of the 3' splice site (3'SS). This pathway furthermore protects most intron-containing nascent transcripts from the effects of cleavage by an intronic hammerhead ribozyme. This suggests that a high percentage of introns are recognized cotranscriptionally. This observation led us to screen a small deletion library for strains that sensitize a splicing reporter to ribozyme cleavage. Characterization of the Deltamud2 strain indicates that the early splicing factor Mud2p functions with U1 snRNP to form a cross-intron bridging complex on nascent pre-mRNA. The complex helps protect the transcript from ribozyme-mediated destruction and suggests an intron-definition event early in the spliceosome assembly process. The transcription elongation mutant strains Deltadst1 and Deltapaf1 show different cotranscriptional splicing phenotypes, suggesting that different transcription pathways differentially impact the efficiency of nascent intron definition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.