ArticleProceedings of the National Academy of Sciences of the United States of America2006
Importance of dosage standardization for interpreting transcriptomal signature profiles: evidence from studies of xenoestrogens.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 70 citations in OpenAlex.
- Molecular response to aromatase inhibitor treatment in primary breast cancer.Breast cancer research : BCR · 2007Trial
- Interference with systemic negative feedback as a potential mechanism for nonmonotonic dose-responses of endocrine-disrupting chemicals.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- Exploring the Complex Mechanisms of Isoflavones: From Cell Bioavailability, to Cell Dynamics and Breast Cancer.Phytotherapy research : PTR · 2025Review
- Microarray analysis of breast cancer gene expression profiling in response to 2-deoxyglucose, metformin, and glucose starvation.Cancer cell international · 2022Article
- Data integration, analysis, and interpretation of eight academic CLARITY-BPA studies.Reproductive toxicology (Elmsford, N.Y.) · 2020Article
- Estrogen receptor 1 expression and methylation ofEnvironmental epigenetics · 2019Article
- Suppression of lymphoma growth by the xenoestrogens bisphenol A and genistein.Endocrine connections · 2018Article
- Article
- BCScreen: A gene panel to test for breast carcinogenesis in chemical safety screening.Computational toxicology (Amsterdam, Netherlands) · 2018Article
- Low dose of Bisphenol A enhance the susceptibility of thyroid carcinoma stimulated by DHPN and iodine excess in F344 rats.Oncotarget · 2017Article
- Editor's Highlight: Transcriptome Profiling Reveals Bisphenol A Alternatives Activate Estrogen Receptor Alpha in Human Breast Cancer Cells.Toxicological sciences : an official journal of the Society of Toxicology · 2017Article
- New insights into fetal mammary gland morphogenesis: differential effects of natural and environmental estrogens.Scientific reports · 2017Article
- Estrogens in the wrong place at the wrong time: Fetal BPA exposure and mammary cancer.Reproductive toxicology (Elmsford, N.Y.) · 2015Review
- Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: the challenge ahead.Carcinogenesis · 2015Review
- Potential therapeutic mechanism of genistein in breast cancer involves inhibition of cell cycle regulation.Molecular medicine reports · 2015Article
- Non-monotonic dose responses in studies of endocrine disrupting chemicals: bisphenol a as a case study.Dose-response : a publication of International Hormesis Society · 2014Article
- Expressomal approach for comprehensive analysis and visualization of ligand sensitivities of xenoestrogen responsive genes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The estrogenic content of rodent diets, bedding, cages, and water bottles and its effect on bisphenol A studies.Journal of the American Association for Laboratory Animal Science : JAALAS · 2013Review
- PPTOX III: environmental stressors in the developmental origins of disease--evidence and mechanisms.Toxicological sciences : an official journal of the Society of Toxicology · 2013Article
- Endocrine-disrupting chemicals and public health protection: a statement of principles from The Endocrine Society.Endocrinology · 2012Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
To obtain insights into similarities and differences in the biological actions of related drugs or toxic agents, their transcriptomal signature profiles (TSPs) have been examined in a large number of studies. However, many such reports did not provide proper justification for the dosage criteria of each agent. Using a well characterized cell culture model of estrogen-dependent proliferation of MCF7 human breast cancer cells, we demonstrate how different approaches to dosage standardization exert critical influences on TSPs, leading to different and even conflicting conclusions. Using quantitative cellular response (QCR)-based dosage criteria, TSPs were determined by Affymetrix microarray when cells were proliferating at comparable rates in the presence of various estrogens. We observed that TSPs of the xenoestrogens (e.g., genistein or bisphenol A) were clearly different from the TSP of 17beta-estradiol; namely, the former strongly enhanced expression of genes involved in mitochondrial oxidative phosphorylation, whereas the latter showed minimal effects. In contrast, TSPs for genistein and 17beta-estradiol were indistinguishable by using the marker gene expression-based dosage criteria, conditions in which there was comparable expression of the mRNA transcripts for the estrogen-inducible WISP2 gene. Our findings indicate that determination and interpretation of TSPs in pharmacogenomic and toxicogenomic studies that examine the transcriptomal actions of related agents by microarray require a clear rationale for the dosage standardization method to be used. We suggest that future studies involving TSP analyses use quantitative and objective dosage standardization methods, such as those with quantitative cellular response or marker gene expression-based dosage criteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.