ArticleRNA (New York, N.Y.)2006
Functional spliceosomal A complexes can be assembled in vitro in the absence of a penta-snRNP.
Article in RNA (New York, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Pathogenic Abnormal Splicing Due to Intronic Deletions that Induce Biophysical Space Constraint for Spliceosome Assembly.American journal of human genetics · 2019Article
- Nuclear cyclophilins affect spliceosome assembly and function in vitro.The Biochemical journal · 2015Article
- Stem-loop 4 of U1 snRNA is essential for splicing and interacts with the U2 snRNP-specific SF3A1 protein during spliceosome assembly.Genes & development · 2014Article
- A day in the life of the spliceosome.Nature reviews. Molecular cell biology · 2014Review
- Antisense-targeted immuno-EM localization of the pre-mRNA path in the spliceosomal C complex.RNA (New York, N.Y.) · 2012Article
- Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands.Nucleic acids research · 2012Article
- Functional mammalian spliceosomal complex E contains SMN complex proteins in addition to U1 and U2 snRNPs.Nucleic acids research · 2012Article
- The DEAD-box RNA helicase-like Utp25 is an SSU processome component.RNA (New York, N.Y.) · 2010Article
- The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells.The Journal of cell biology · 2010Article
- The intronic splicing code: multiple factors involved in ATM pseudoexon definition.The EMBO journal · 2010Article
- Exon, intron and splice site locations in the spliceosomal B complex.The EMBO journal · 2009Article
- Assembly of ribosomes and spliceosomes: complex ribonucleoprotein machines.Current opinion in cell biology · 2009Review
- Stalling of spliceosome assembly at distinct stages by small-molecule inhibitors of protein acetylation and deacetylation.RNA (New York, N.Y.) · 2009Article
- The assembly of a spliceosomal small nuclear ribonucleoprotein particle.Nucleic acids research · 2008Review
- "Nought may endure but mutability": spliceosome dynamics and the regulation of splicing.Molecular cell · 2008Review
- Mutation in the splicing factor Hprp3p linked to retinitis pigmentosa impairs interactions within the U4/U6 snRNP complex.Human molecular genetics · 2008Article
- Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes.The EMBO journal · 2007Article
- A genome-wide analysis indicates that yeast pre-mRNA splicing is predominantly posttranscriptional.Molecular cell · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two different models currently exist for the assembly pathway of the spliceosome, namely, the traditional model, in which spliceosomal snRNPs associate in a stepwise, ordered manner with the pre-mRNA, and the holospliceosome model, in which all spliceosomal snRNPs preassemble into a penta-snRNP complex. Here we have tested whether the spliceosomal A complex, which contains solely U1 and U2 snRNPs bound to pre-mRNA, is a functional, bona fide assembly intermediate. Significantly, A complexes affinity-purified from nuclear extract depleted of U4/U6 snRNPs (and thus unable to form a penta-snRNP) supported pre-mRNA splicing in nuclear extract depleted of U2 snRNPs, whereas naked pre-mRNA did not. Mixing experiments with purified A complexes and naked pre-mRNA additionally confirmed that under these conditions, A complexes do not form de novo. Thus, our studies demonstrate that holospliceosome formation is not a prerequisite for generating catalytically active spliceosomes and that, at least in vitro, the U1 and U2 snRNPs can functionally associate with the pre-mRNA, prior to and independent of the tri-snRNP. The ability to isolate functional spliceosomal A complexes paves the way to study in detail subsequent spliceosome assembly steps using purified components.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.