ArticleMolecular and cellular biology2006
Expression of rRNA genes and nucleolus formation at ectopic chromosomal sites in the yeast Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Additional principles that govern the release of pre-ribosomes from the nucleolus into the nucleoplasm in yeast.Nucleic acids research · 2023Article
- Yeast ribosome biogenesis factors Puf6 and Nog2 and ribosomal proteins uL2 and eL43 act in concert to facilitate the release of nascent large ribosomal subunits from the nucleolus.Nucleic acids research · 2023Article
- The rDNA is biomolecular condensate formed by polymer-polymer phase separation and is sequestered in the nucleolus by transcription and R-loops.Nucleic acids research · 2021Article
- Age-Dependent Ribosomal DNA Variations in Mice.Molecular and cellular biology · 2020Article
- Nuclear microenvironment in cancer: Control through liquid-liquid phase separation.Cancer science · 2020Review
- NORs on human acrocentric chromosome p-arms are active by default and can associate with nucleoli independently of rDNA.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Biological phase separation: cell biology meets biophysics.Biophysical reviews · 2020Review
- Compositional reorganization of the nucleolus in budding yeast mitosis.Molecular biology of the cell · 2019Article
- Article
- Enrichment of dynamic chromosomal crosslinks drive phase separation of the nucleolus.Nucleic acids research · 2017Article
- Decoding the principles underlying the frequency of association with nucleoli for RNA polymerase III-transcribed genes in budding yeast.Molecular biology of the cell · 2016Article
- Resolving complex chromosome structures during meiosis: versatile deployment of Smc5/6.Chromosoma · 2016Review
- Determinants of mammalian nucleolar architecture.Chromosoma · 2015Review
- Construction of synthetic nucleoli in human cells reveals how a major functional nuclear domain is formed and propagated through cell division.Genes & development · 2014Article
- The nucleolus: a raft adrift in the nuclear sea or the keystone in nuclear structure?Biomolecular concepts · 2013Review
- Review
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5 authors.
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Abstract
We constructed yeast strains in which rRNA gene repeats are integrated at ectopic sites in the presence or absence of the native nucleolus. At all three ectopic sites analyzed, near centromere CEN5, near the telomere of chromosome VI-R, and in middle of chromosome V-R (mid-V-R), a functional nucleolus was formed, and no difference in the expression of rRNA genes was observed. When two ribosomal DNA (rDNA) arrays are present, one native and the other ectopic, there is codominance in polymerase I (Pol I) transcription. We also examined the expression of a single rDNA repeat integrated into ectopic loci in strains with or without the native RDN1 locus. In a strain with reduced rRNA gene copies at RDN1 (approximately 40 copies), the expression of a single rRNA gene copy near the telomere was significantly reduced relative to the other ectopic sites, suggesting a less-efficient recruitment of the Pol I machinery from the RDN1 locus. In addition, we found a single rRNA gene at mid-V-R was as active as that within the 40-copy RDN1. Combined with the results of activity analysis of a single versus two tandem copies at CEN5, we conclude that tandem repetition is not required for efficient rRNA gene transcription.
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