ArticleMolecular and cellular biology2006
Transcriptional pausing caused by NELF plays a dual role in regulating immediate-early expression of the junB gene.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
67 citing papers in PubMed, 99 citations in OpenAlex.
- eIF3i facilitates NELFCD translation to promote metastasis via regulating EMT and invadopodia.British journal of cancer · 2026Article
- HSV-1 Infection Induces a Downstream Shift of Promoter-Proximal Pausing for Host Genes.Journal of virology · 2023Article
- ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II.Nature communications · 2022Article
- Nucleotide stress responses in neural crest cell fate and melanoma.Cell cycle (Georgetown, Tex.) · 2021Review
- NELF Regulates a Promoter-Proximal Step Distinct from RNA Pol II Pause-Release.Molecular cell · 2020Article
- Article
- Transcription pausing regulates mouse embryonic stem cell differentiation.Stem cell research · 2017Article
- Transcriptional response to stress is pre-wired by promoter and enhancer architecture.Nature communications · 2017Article
- Dynamic Change of Transcription Pausing through Modulating NELF Protein Stability Regulates Granulocytic Differentiation.Blood advances · 2017Article
- Pause & go: from the discovery of RNA polymerase pausing to its functional implications.Current opinion in cell biology · 2017Review
- Article
- Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.Nucleic acids research · 2016Article
- Article
- Ups and Downs of Poised RNA Polymerase II in B-Cells.PLoS computational biology · 2016Article
- Mitogen-activated protein kinase signaling controls basal and oncostatin M-mediated JUNB gene expression.Molecular and cellular biochemistry · 2015Article
- Retroviral cyclin controls cyclin-dependent kinase 8-mediated transcription elongation and reinitiation.Journal of virology · 2015Article
- The Integrator complex controls the termination of transcription at diverse classes of gene targets.Cell research · 2015Article
- Hormone-induced and DNA demethylation-induced relief of a tissue-specific and developmentally regulated block in transcriptional elongation.The Journal of biological chemistry · 2014Article
- A kinase-independent activity of Cdk9 modulates glucocorticoid receptor-mediated gene induction.Biochemistry · 2014Article
- A conserved protein motif is required for full modulatory activity of negative elongation factor subunits NELF-A and NELF-B in modifying glucocorticoid receptor-regulated gene induction properties.The Journal of biological chemistry · 2013Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) and negative elongation factor (NELF) negatively regulate transcription elongation by RNA polymerase II (RNAPII) in vitro. However, the physiological roles of this negative regulation are not well understood. Here, by using a number of approaches to identify protein-DNA interactions in vivo, we show that DSIF- and NELF-mediated transcriptional pausing has a dual function in regulating immediate-early expression of the human junB gene. Before induction by interleukin-6, RNAPII, DSIF, and NELF accumulate in the promoter-proximal region of junB, mainly at around position +50 from the transcription initiation site. After induction, the association of these proteins with the promoter-proximal region continues whereas RNAPII and DSIF are also found in the downstream regions. Depletion of a subunit of NELF by RNA interference enhances the junB mRNA level both before and after induction, indicating that DSIF- and NELF-mediated pausing contributes to the negative regulation of junB expression, not only by inducing RNAPII pausing before induction but also by attenuating transcription after induction. These regulatory mechanisms appear to be conserved in other immediate-early genes as well.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.