Evidence map›Paper›PMID 16880387›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2006

Clonogenic multipotent stem cells in human adipose tissue differentiate into functional smooth muscle cells.

Larissa V Rodríguez, Zeni Alfonso, Rong Zhang, Joanne Leung, Benjamin Wu, Louis J Ignarro

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
112citing papers in PubMed, 1 pooled it
8.8field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

112 citing papers in PubMed, 1 synthesis or guideline pooled it, 316 citations in OpenAlex.

  1. Osteochondral tissue engineering approaches for articular cartilage and subchondral bone regeneration.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2012
    Pooled it
  2. Trial
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  6. Article
  7. Soft Tissue Reconstruction.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
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  11. Overexpression of integrin βAnnals of translational medicine · 2022
    Article
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52 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Larissa V RodríguezDepartment of Urology, University of California School of Medicine, Los Angeles, CA 90024, USA. lrodriguez@mednet.ucla.edu
Zeni Alfonso
Rong Zhang
Joanne Leung
Benjamin Wu
Louis J Ignarro
University of California, Los Angeles · US

Funding

Building Interdisciplinary Research Careers in Women's Health (BIRCWH) CenterK12HD001400 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHAUDHURI, GAUTAM · 2000 to 2009
$4.2M
ADIPOSE DERIVED STEM CELLS FOR TREATMENT OF INCONTINENCER01DK067198 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI RODRIGUEZ, LARISSA · 2004 to 2008
$1.8M
NICHD NIH HHS 5-K12-HD01400NICHD NIH HHS K12 HD001400NIDDK NIH HHS R01 DK067198NIDDK NIH HHS R01 DK067198-01
6 · The paper itself

Abstract

Smooth muscle is a major component of human tissues and is essential for the normal function of a multitude of organs including the intestine, urinary tract and the vascular system. The use of stem cells for cell-based tissue engineering and regeneration strategies represents a promising alternative for smooth muscle repair. For such strategies to succeed, a reliable source of smooth muscle precursor cells must be identified. Adipose tissue provides an abundant source of multipotent cells. In this study, the capacity of processed lipoaspirate (PLA) and adipose-derived stem cells to differentiate into phenotypic and functional smooth muscle cells was evaluated. To induce differentiation, PLA cells were cultured in smooth muscle differentiation medium. Smooth muscle differentiation of PLA cells induced genetic expression of all smooth muscle markers and further confirmed by increased protein expression of smooth muscle cell-specific alpha actin (ASMA), calponin, caldesmon, SM22, myosin heavy chain (MHC), and smoothelin. Clonal studies of adipose derived multipotent cells demonstrated differentiation of these cells into smooth muscle cells in addition to trilineage differentiation capacity. Importantly, smooth muscle-differentiated cells, but not their precursors, exhibit the functional ability to contract and relax in direct response to pharmacologic agents. In conclusion, adipose-derived cells have the potential to differentiate into functional smooth muscle cells and, thus, adipose tissue can be a useful source of cells for treatment of injured tissues where smooth muscle plays an important role.

Indexed as

Cell DifferentiationGene Expression RegulationActinsAdipose TissueAnimalsCalcium-Binding ProteinsCalmodulin-Binding ProteinsCalponinsHumansMicrofilament ProteinsMuscle, SmoothMyocytes, Smooth MuscleMyosin Heavy ChainsRatsRats, Sprague-DawleyActinsCalcium-Binding ProteinsCalmodulin-Binding ProteinsCalponinsMicrofilament ProteinsMyosin Heavy Chains

Identifiers

PMID16880387
PMCPMC1567713
OpenAlexW2021202906

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.