ArticleSao Paulo medical journal = Revista paulista de medicina2006
Model for human skin reconstructed in vitro composed of associated dermis and epidermis.
Article in Sao Paulo medical journal = Revista paulista de medicina, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 70 citations in OpenAlex.
- In Vitro and Ex Vivo Evaluation of Rifampicin Cytotoxicity in Human Skin Models.Antibiotics (Basel, Switzerland) · 2025Article
- Advanced function, design and application of skin substitutes for skin regeneration.Materials today. Bio · 2024Review
- Current and Future Perspectives on Skin Tissue Engineering: Key Features of Biomedical Research, Translational Assessment, and Clinical Application.Advanced healthcare materials · 2019Review
- The effect of aging in primary human dermal fibroblasts.PloS one · 2019Article
- Epithelial cells from oral mucosa: How to cultivate them?Cytotechnology · 2016Article
- Modeling Barrier Tissues In Vitro: Methods, Achievements, and Challenges.EBioMedicine · 2016Review
- Vital roles of stem cells and biomaterials in skin tissue engineering.World journal of stem cells · 2015Review
- Design and fabrication of human skin by three-dimensional bioprinting.Tissue engineering. Part C, Methods · 2014Article
- Key regulatory role of dermal fibroblasts in pigmentation as demonstrated using a reconstructed skin model: impact of photo-aging.PloS one · 2014Article
- Fetal wound healing biomarkers.Disease markers · 2013Review
- Article
- Immunoarchitectural characterization of a human skin model reconstructed in vitro.Sao Paulo medical journal = Revista paulista de medicina · 2009Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CONTEXT AND
objectiveThe technique of obtaining human skin with dermis and epidermis reconstructed from cells isolated from patients can enable autologous skin grafting on patients with few donor sites. It also enables in vitro trials on chemicals and drugs. The objective of this work was to demonstrate a method for obtaining human skin composed of associated dermis and epidermis, reconstructed in vitro. DESIGN AND
settingExperimental laboratory study, in the Skin Cell Culture Laboratory of Faculdade de Ciências Médicas, Universidade Estadual de Campinas.
methodsCells from human fibroblast cultures are injected into bovine collagen type I matrix and kept immersed in specific culturing medium for fibroblasts. This enables human dermis reconstruction in vitro. On this, by culturing human keratinocytes and melanocytes, differentiated epidermis is formed, leading to the creation of human skin composed of associated dermis and epidermis, reconstructed in vitro.
resultsWe showed that human skin composed of associated dermis and epidermis can be successfully reconstructed in vitro. It is histologically formed in the same way as human skin in vivo. Collagen tissue can be identified in the dermis, with cells and extracellular matrix organized in parallel to multilayer epidermis.
conclusionsIt is possible to obtain completely differentiated human skin composed of associated dermis and epidermis, reconstructed in vitro, from injection of human fibroblasts into bovine collagen type I matrix and culturing of human keratinocytes and melanocytes on this matrix.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.