ArticleEMBO reports2006
Assembly of transcription factor IIB at a promoter in vivo requires contact with RNA polymerase II.
Article in EMBO reports, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- A specialized TFIIB is required for transcription of transposon-targeting noncoding RNAs.Nucleic acids research · 2025Article
- Integrative bioinformatics analysis reveals STAT1, ORC2, and GTF2B as critical biomarkers in lupus nephritis with Monkeypox virus infection.Scientific reports · 2025Article
- Transcription factor GTF2B regulates AIP protein expression in growth hormone-secreting pituitary adenomas and influences tumor phenotypes.Neuro-oncology · 2022Article
- A novel method to investigate the effects of gene mutations at the cellular level using a dual expression lentiviral vector.Bioscience reports · 2019Article
- Rapid dynamics of general transcription factor TFIIB binding during preinitiation complex assembly revealed by single-molecule analysis.Genes & development · 2016Article
- TFIIB is only ∼9 Å away from the 5'-end of a trimeric RNA primer in a functional RNA polymerase II preinitiation complex.PloS one · 2015Article
- The methyltransferase WBSCR22/Merm1 enhances glucocorticoid receptor function and is regulated in lung inflammation and cancer.The Journal of biological chemistry · 2014Article
- A new paradigm for transcription factor TFIIB functionality.Scientific reports · 2014Article
- The RNA polymerase II preinitiation complex. Through what pathway is the complex assembled?Transcription · 2014Review
- TFIIB dephosphorylation links transcription inhibition with the p53-dependent DNA damage response.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The linker domain of basal transcription factor TFIIB controls distinct recruitment and transcription stimulation functions.Nucleic acids research · 2011Article
- Phosphorylation of TFIIB links transcription initiation and termination.Current biology : CB · 2010Article
- Hypoxia and glucocorticoid signaling converge to regulate macrophage migration inhibitory factor gene expression.Arthritis and rheumatism · 2009Article
- TFIIB recognition elements control the TFIIA-NC2 axis in transcriptional regulation.Molecular and cellular biology · 2009Article
- Dynamic interaction between WT1 and BASP1 in transcriptional regulation during differentiation.Nucleic acids research · 2009Article
- TFIIB and the regulation of transcription by RNA polymerase II.Chromosoma · 2007Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The general transcription factor TFIIB has a central role in the assembly of the preinitiation complex at the promoter, providing a platform for the entry of RNA polymerase II/TFIIF. We used an RNA interference (RNAi)-based system in which TFIIB expression is ablated in vivo and replaced with a TFIIB derivative that contains a silent mutation and is refractory to the RNAi. Using this approach, we found that transcriptionally defective TFIIB amino-terminal mutants showed distinct effects on the basis of their ability to compete with wild-type TFIIB in vivo. Moreover, analysis of the TFIIB mutant derivatives by chromatin immunoprecipitation showed that promoter occupancy by TFIIB is dependent on the association with RNA polymerase II. Together, our results support a mode of preinitiation complex assembly in which TFIIB/RNA polymerase II recruitment to the promoter occurs in vivo.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.