ArticleJournal of the American Society for Mass Spectrometry2006
RNA fragmentation in MALDI mass spectrometry studied by H/D-exchange: mechanisms of general applicability to nucleic acids.
Article in Journal of the American Society for Mass Spectrometry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- A High-Throughput Workflow for Mass Spectrometry Analysis of Nucleic Acids by Nanoflow Desalting.Analytical chemistry · 2024Article
- In-Gel Cyanoethylation for Pseudouridines Mass Spectrometry Detection of Bacterial Regulatory RNA.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Review of fragmentation of synthetic single-stranded oligonucleotides by tandem mass spectrometry from 2014 to 2022.Rapid communications in mass spectrometry : RCM · 2023Review
- Studying the Key Intermediate of RNA Autohydrolysis by Cryogenic Gas-Phase Infrared Spectroscopy.Angewandte Chemie (International ed. in English) · 2022Article
- The effect of adenine protonation on RNA phosphodiester backbone bond cleavage elucidated by deaza-nucleobase modifications and mass spectrometry.Nucleic acids research · 2019Article
- Article
- Label-free, direct localization and relative quantitation of the RNA nucleobase methylations m6A, m5C, m3U, and m5U by top-down mass spectrometry.Nucleic acids research · 2017Article
- Catalytic metallodrugs based on the LaR2C peptide target HCV SLIV IRES RNA.Dalton transactions (Cambridge, England : 2003) · 2015Article
- On the mechanism of RNA phosphodiester backbone cleavage in the absence of solvent.Nucleic acids research · 2015Article
- More than charged base loss--revisiting the fragmentation of highly charged oligonucleotides.Journal of the American Society for Mass Spectrometry · 2014Article
- Insight into the recognition, binding, and reactivity of catalytic metallodrugs targeting stem loop IIb of hepatitis C IRES RNA.ChemMedChem · 2014Article
- Quantitative analysis of rRNA modifications using stable isotope labeling and mass spectrometry.Journal of the American Chemical Society · 2014Article
- Gas-phase dissociation of homo-DNA oligonucleotides.Journal of the American Society for Mass Spectrometry · 2013Article
- Kinetics and Mechanisms of Oxidative Cleavage of HIV RRE RNA by Rev-Coupled Transition Metal Chelates.Chemical science · 2013Article
- OMA and OPA--software-supported mass spectra analysis of native and modified nucleic acids.Journal of the American Society for Mass Spectrometry · 2013Article
- Analysis of RNA cleavage by MALDI-TOF mass spectrometry.Nucleic acids research · 2013Article
- Enhanced in-source fragmentation in MALDI-TOF-MS of oligonucleotides using 1,5-diaminonapthalene.Journal of the American Society for Mass Spectrometry · 2012Article
- Tandem mass spectrometry of modified and platinated oligoribonucleotides.Journal of the American Society for Mass Spectrometry · 2011Article
- ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA.Nature communications · 2011Article
- Top-down tandem mass spectrometry of tRNA via ion trap collision-induced dissociation.Journal of the American Society for Mass Spectrometry · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To reveal the gas-phase chemistry of RNA and DNA fragmentation during MALDI mass spectrometry in positive ion mode, we performed hydrogen/deuterium exchange on a series of RNA and DNA tetranucleotides and studied their fragmentation patterns on a high-resolution MALDI TOF-TOF instrument. We were specifically interested in elucidating the remarkably different fragmentation behavior of RNA and DNA, i.e., the characteristic and abundant production of c- and y-ions from RNA versus a dominating generation of (a-B)- and w-ions from DNA analytes. The analysis yielded important information on all significant backbone cleavages as well as nucleobase losses. Based on this, we suggest common fragmentation mechanisms for RNA and DNA as well as an important RNA-specific reaction requiring a 2'-hydroxyl group, leading to c- and y-ions. The data is viewed and discussed in the context of previously published data to obtain a coherent picture of the fragmentation of singly protonated nucleic acids.
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Registered trials
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