ArticleProceedings of the National Academy of Sciences of the United States of America2006
The cotton fiber zinc-binding domain of cellulose synthase A1 from Gossypium hirsutum displays rapid turnover in vitro and in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 81 citations in OpenAlex.
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- The plant cell wall-dynamic, strong, and adaptable-is a natural shapeshifter.The Plant cell · 2024Review
- The Regulation of Cellulose Biosynthesis in Plants.The Plant cell · 2019Review
- Domain swaps of Arabidopsis secondary wall cellulose synthases to elucidate their class specificity.Plant direct · 2018Article
- The cell biology of secondary cell wall biosynthesis.Annals of botany · 2018Review
- Longevity in vivo of primary cell wall cellulose synthases.Plant molecular biology · 2018Article
- MicroRNA expression profiles during cotton (Gossypium hirsutum L) fiber early development.Scientific reports · 2017Article
- Transcriptome portrait of cellulose-enriched flax fibres at advanced stage of specialization.Plant molecular biology · 2017Article
- Differential expression of microRNAs during fiber development between fuzzless-lintless mutant and its wild-type allotetraploid cotton.Scientific reports · 2017Article
- The DEK1 Calpain Linker Functions in Three-Dimensional Body Patterning in Physcomitrella patens.Plant physiology · 2016Article
- Proteomic profiling of cellulase-aid-extracted membrane proteins for functional identification of cellulose synthase complexes and their potential associated- components in cotton fibers.Scientific reports · 2016Article
- Systems and synthetic biology approaches to alter plant cell walls and reduce biomass recalcitrance.Plant biotechnology journal · 2014Review
- Cellulose synthesis and its regulation.The arabidopsis book · 2014Article
- Patterning and lifetime of plasma membrane-localized cellulose synthase is dependent on actin organization in Arabidopsis interphase cells.Plant physiology · 2013Article
- Article
- The UDP-glucuronate decarboxylase gene family in Populus: structure, expression, and association genetics.PloS one · 2013Article
- Analysis of cellulose synthase genes from domesticated apple identifies collinear genes WDR53 and CesA8A: partial co-expression, bicistronic mRNA, and alternative splicing of CESA8A.Journal of experimental botany · 2012Article
- In vitro inhibition of pigmentation and fiber development in colored cotton.Journal of Zhejiang University. Science. B · 2012Article
- Chemical genetics to examine cellulose biosynthesis.Frontiers in plant science · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Little is known about the assembly and turnover of cellulose synthase complexes commonly called rosettes. Recent work indicates that rosette assembly could involve the dimerization of CesA (cellulose synthase catalytic subunit) proteins regulated by the redox state of the CesA zinc-binding domain (ZnBD). Several studies in the 1980s led to the suggestion that synthase complexes may have very short half-lives in vivo, but no recent work has directly addressed this issue. In the present work, we show that the half-life of cotton fiber GhCesA1 protein is <30 min in vivo, far less than the average membrane protein. We also show that the reduced monomer of GhCesA1 ZnBD is rapidly degraded when exposed to cotton fiber extracts, whereas the oxidized dimer is resistant to degradation. Low rates of degradation activity were detected in vitro by using extracts from fibers harvested during primary cell-wall formation, but activity increased markedly during transition to secondary cell-wall synthesis. In vitro degradation of reduced GhCesA1 ZnBD is inhibited by proteosome inhibitor MG132 and also by E64 and EGTA, suggesting that proteolysis is initiated by cysteine protease activity rather than the proteosome. We used a yeast two-hybrid system to identify a putative cotton fiber metallothionein and to confirm it as a protein that could interact with the GhCesA1 ZnBD. A model is proposed wherein active cellulose synthase complexes contain CesA proteins in dimerized form, and turnover and degradation of the complexes are mediated through reductive zinc insertion by metallothionein and subsequent proteolysis involving a cysteine protease.
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