ArticleJournal of the American Society for Mass Spectrometry2006
Using metal-catalyzed oxidation reactions and mass spectrometry to identify amino acid residues within 10 A of the metal in Cu-binding proteins.
Article in Journal of the American Society for Mass Spectrometry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Article
- Effect of the metal ion-induced carbonylation modification of mitochondrial membrane channel protein VDAC on cell vitality, seedling growth and seed aging.Frontiers in plant science · 2023Article
- MEMBRANE PROTEIN STRUCTURES AND INTERACTIONS FROM COVALENT LABELING COUPLED WITH MASS SPECTROMETRY.Mass spectrometry reviews · 2022Review
- Just how prevalent are peptide therapeutic products? A critical review.International journal of pharmaceutics · 2020Review
- Determination of the glycoprotein specificity of lectins on cell membranes through oxidative proteomics.Chemical science · 2020Article
- Mass Spectrometry-Based Protein Footprinting for Higher-Order Structure Analysis: Fundamentals and Applications.Chemical reviews · 2020Review
- Covalent labeling-mass spectrometry with non-specific reagents for studying protein structure and interactions.Methods (San Diego, Calif.) · 2018Review
- Reactive oxygen species leave a damage trail that reveals water channels in Photosystem II.Science advances · 2017Article
- Article
- Unique effect of Cu(II) in the metal-induced amyloid formation of β-2-microglobulin.Biochemistry · 2014Article
- Amino acid influence on copper binding to peptides: cysteine versus arginine.Journal of the American Society for Mass Spectrometry · 2010Article
- Divalent metal ions in plant mitochondria and their role in interactions with proteins and oxidative stress-induced damage to respiratory function.Plant physiology · 2010Article
- Copper binding to beta-2-microglobulin and its pre-amyloid oligomers.Biochemistry · 2009Article
- Copper binding extrinsic to the octarepeat region in the prion protein.Current protein & peptide science · 2009Review
- Correct identification of oxidized histidine residues using electron-transfer dissociation.Journal of mass spectrometry : JMS · 2009Article
- Pulsed electron beam water radiolysis for submicrosecond hydroxyl radical protein footprinting.Analytical chemistry · 2009Article
- Using mass spectrometry-based methods to understand amyloid formation and inhibition of alpha-synuclein and amyloid beta.Mass spectrometry reviewsReview
- A Robust Analytical Approach for the Identification of Specific Protein Carbonylation Sites: Metal-Catalyzed Oxidations of Human Serum Albumin.Analytical lettersArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Metal-catalyzed oxidation (MCO) reactions and mass spectrometry (MS) can be used together to determine the amino acids bound to Cu in a metalloprotein. Selective oxidation of only amino acids bound to Cu during the MCO/MS approach relies on proper choice of the types and concentrations of the reducing and oxidizing agents. We show here that if these MCO reagent concentrations are "detuned" or varied slightly from optimal conditions, nonmetal-bound amino acids close to Cu can also be oxidized in a controlled manner. Using Cu/Zn superoxide dismutase as a model system, we demonstrate that amino acids up to 10 A from Cu can be modified as long as they are readily accessible to diffusing reactive oxygen species. UV/VIS spectroscopy and measurements of oxidation kinetics provide evidence that the protein's structural integrity around Cu is maintained during the detuned MCO reactions. Because this method can identify amino acids around Cu that have low solvent accessibility, it should complement other radical-based protein surface-mapping techniques.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.