ArticleSaline systems2006
NhaD type sodium/proton-antiporter of Halomonas elongata: a salt stress response mechanism in marine habitats?
Article in Saline systems, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
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- ABC-type salt tolerance transporter genes are abundant and mutually shared among the microorganisms of the hypersaline Sambhar Lake.Extremophiles : life under extreme conditions · 2025Article
- Culture-supported ecophysiology of the SAR116 clade demonstrates metabolic and spatial niche partitioning.The ISME journal · 2025Article
- Evolutionary engineering and molecular characterization of cobalt-resistantFrontiers in microbiology · 2024Article
- Adaptation to Varying Salinity inFrontiers in microbiology · 2022Article
- Population genomics of Vibrionaceae isolated from an endangered oasis reveals local adaptation after an environmental perturbation.BMC genomics · 2020Article
- Draft Genome Sequence of Halomonas urumqiensis BZ-SZ-XJ27Microbiology resource announcements · 2018Article
- Article
- Characterization of a Functionally Unknown Arginine-Aspartate-Aspartate Family Protein FromFrontiers in microbiology · 2018Article
- Critical Functions of Region 1-67 and Helix XIII in Retaining the Active Structure of NhaD Antiporter inFrontiers in microbiology · 2018Article
- Functional Interaction between the N and C Termini of NhaD Antiporters from Halomonas sp. Strain Y2.Journal of bacteriology · 2017Article
- Characterization of a novel two-component NaScientific reports · 2017Article
- A UPF0118 family protein with uncharacterized function from the moderate halophile Halobacillus andaensis represents a novel class of NaScientific reports · 2017Article
- Competition is the basis of the transport mechanism of the NhaB Na+/H+ exchanger from Klebsiella pneumoniae.PloS one · 2017Article
- Alkaline Response of a Halotolerant Alkaliphilic Halomonas Strain and Functional Diversity of Its Na+(K+)/H+ Antiporters.The Journal of biological chemistry · 2016Article
- Expression and functional analysis of two NhaD type antiporters from the halotolerant and alkaliphilic Halomonas sp. Y2.Extremophiles : life under extreme conditions · 2016Article
- Single cells within the Puerto Rico trench suggest hadal adaptation of microbial lineages.Applied and environmental microbiology · 2015Article
- Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis.Extremophiles : life under extreme conditions · 2014Article
- Glutamine synthetase 2 is not essential for biosynthesis of compatible solutes in Halobacillus halophilus.Frontiers in microbiology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSodium/proton-antiporters (Nha) are known to play an important role in pH- and Na+-homeostasis. In microorganisms several types with different capacity, affinity and selectivity for Na+ and Li+ exist. The homeostasis system of E. coli, NhaA and NhaB, is well researched, but the function of other types of Na+/H+-antiporters like NhaD is yet to be fully understood. Since several antiporters play an important role at various points in the physiology of higher organisms, one can speculate that the main functions of some of those procaryotic antiporters differ from pH- and Na+-homeostasis.
resultsThis study investigates the function and regulation of a gene encoding for a NhaD type antiporter which was discovered in the halophilic eubacterium Halomonas elongata. The deduced primary amino acid sequence of the abovementioned gene showed more than 60% identity to known antiporters of the NhaD type from Alkalimonas amylolytica, Shewanella oneidensis and several other marine organisms of the gamma-Proteobacteria. Evidence was found for a dual regulation of H. elongata NhaD expression. The gene was cloned and expressed in E. coli. Antiporter deficient NaCl and LiCl sensitive E. coli mutants EP432 and KNabc were partially complemented by a plasmid carrying the H. elongata nhaD gene. Surprisingly the LiCl sensitivity of E. coli strain DH5alpha having a complete homeostasis system was increased when NhaD was co-expressed.
conclusionSince NhaD is an antiporter known so far only from halophilic or haloalcaliphilic Proteobacteria one can speculate that this type of antiporter provides a special mechanism for adaptation to marine habitats. As was already speculated--though without supporting data--and substantiated in this study this might be active Na+-import for osmoregulatory purposes.
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