ArticleNucleic acids research2006
De novo assembly of genuine replication forks on an immobilized circular plasmid in Xenopus egg extracts.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 10 citations in OpenAlex.
- Different roles of the human Orc6 protein in the replication initiation process.Cellular and molecular life sciences : CMLS · 2011Article
- The DNA unwinding element binding protein DUE-B interacts with Cdc45 in preinitiation complex formation.Molecular and cellular biology · 2010Article
- Detection of Schistosoma mansoni eggs in feces through their interaction with paramagnetic beads in a magnetic field.PLoS neglected tropical diseases · 2007Article
- DNA structure-induced recruitment and activation of the Fanconi anemia pathway protein FANCD2.Molecular and cellular biology · 2007Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe an improved model of DNA replication in Xenopus egg extracts, in which a circular plasmid immobilized on paramagnetic beads is used as a template. DNA synthesis occurred on either circular or linear plasmids coupled to the beads, but only DNA synthesis on the circular plasmid was inhibited by geminin and a CDK inhibitor, p21. DNA synthesis on the circular plasmid occurred after a time lag, during which nuclear formation was probably occurring. Although pre-replicative complexes (pre-RCs) were formed soon after mixing plasmids with egg extracts, binding of CDC45, RPA, Pol alpha, delta and epsilon, and PCNA to the circular plasmid was delayed, but still correlated with DNA synthesis. Moreover, p21 inhibited binding of these replication fork proteins to the circular plasmid. Therefore, the circular plasmid, but not the linear plasmid, assembles bona fide replication forks in egg extracts. We conclude that this improved replication system will be useful for studying the mechanism of formation of replication forks in eukaryotic DNA replication.
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