ArticlePlanta2006
Purification and characterization of a calcium-dependent protein kinase from beetroot plasma membranes.
Article in Planta, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 24 citations in OpenAlex.
- Voltage-Dependent Anion-Selective Channels and Other Mitochondrial Membrane Proteins Form Diverse Complexes in Beetroots Subjected to Flood-Induced Programmed Cell Death.Frontiers in plant science · 2021Article
- Cardiolipin deficiency causes a dissociation of the b 6 c:caa 3 megacomplex in B. subtilis membranes.Journal of bioenergetics and biomembranes · 2016Article
- Article
- Ca(2+) monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor.Scientific reports · 2016Article
- Regulation of the plasma membrane proton pump (H(+)-ATPase) by phosphorylation.Current opinion in plant biology · 2015Review
- Article
- The composition of the Bacillus subtilis aerobic respiratory chain supercomplexes.Journal of bioenergetics and biomembranes · 2012Article
- Short distance movement of genomic negative strands in a host and nonhost for Sugarcane mosaic virus (SCMV).Virology journal · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several calcium-dependent protein kinases (CDPKs) are located in plant plasma membranes where they phosphorylate enzymes and transporters, like the H(+)-ATPase and water channels, thereby regulating their activities. In order to determine which kinases phosphorylate the H(+)-ATPase, a calcium-dependent kinase was purified from beetroot (Beta vulgaris L.) plasma membranes by anion-exchange chromatography, centrifugation in glycerol gradients and hydrophobic interaction chromatography. The kinetic parameters of this kinase were determined (V(max): 3.5 micromol mg(-1) min(-1), K(m) for ATP: 67 microM, K(m) for syntide 2: 15 microM). The kinase showed an optimum pH of 6.8 and a marked dependence on low-micromolar Ca(2+) concentrations (K(d): 0.77 microM). During the purification procedure, a 63-kDa protein with an isoelectric point of 4.7 was enriched. However, this protein was shown not to be a kinase by mass spectrometry. Kinase activity gels showed that a 50-kDa protein could be responsible for most of the activity in purified kinase preparations. This protein was confirmed to be a CDPK by mass spectrometry, possibly the red beet ortholog of rice CDPK2 and Arabidopsis thaliana CPK9, both found associated with membranes. This kinase was able to phosphorylate purified H(+)-ATPase in a Ca(2+)-dependent manner.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.