ArticleJournal of feline medicine and surgery2007
Evaluation of a novel nested PCR for the routine diagnosis of feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV).
Article in Journal of feline medicine and surgery, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- WSAVA Guidelines for the vaccination of dogs and cats.The Journal of small animal practice · 2016Guideline
- Innovative Colorimetric Neutral Red-Based Loop-Mediated Isothermal Amplification (NR-LAMP) Assay: Transforming Rapid and Affordable Feline Leukemia Virus Detection.International journal of molecular sciences · 2025Article
- Molecular Detection of Vector-Borne Pathogens and Their Association with Feline Immunodeficiency Virus and Feline Leukemia Virus in Cats from Northeastern Thailand.Animals : an open access journal from MDPI · 2025Article
- An exceptional case of peritoneal cystic echinococcosis in a domestic cat in Australia.Australian veterinary journal · 2025Article
- Antimicrobial Susceptibility of CommensalAnimals : an open access journal from MDPI · 2024Article
- The detection and full genomic characterization of domestic cat Orthohepadnaviruses from Türkiye.Veterinary medicine and science · 2023Article
- Molecular characterization of Brazilian FeLV strains in São Luis, Maranhão Brazil.Virus genes · 2023Article
- Rapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (RPA) and reverse transcriptase-RPA.Scientific reports · 2021Article
- Comparative measurement of FeLV load in hemolymphatic tissues of cats with hematologic cytopenias.BMC veterinary research · 2019Article
- Follow-Up of Viral Parameters in FeLV- or FIV-Naturally Infected Cats Treated Orally with Low Doses of Human Interferon Alpha.Viruses · 2019Article
- Prospects in Innate Immune Responses as Potential Control Strategies against Non-Primate Lentiviruses.Viruses · 2018Review
- Comparison of three blood transfusion guidelines applied to 31 feline donors to minimise the risk of transfusion-transmissible infections.Journal of feline medicine and surgery · 2018Article
- Genotyping of feline leukemia virus in Mexican housecats.Archives of virology · 2016Article
- Development and clinical evaluation of a rapid diagnostic kit for feline leukemia virus infection.Journal of veterinary science · 2014Article
- Molecular and clinical study on prevalence of feline herpesvirus type 1 and calicivirus in correlation with feline leukemia and immunodeficiency viruses.Veterinary research forum : an international quarterly journal · 2014Article
- Prevalence of feline leukemia virus infection in domestic cats in Rio de Janeiro.Journal of feline medicine and surgery · 2012Article
- Characterization of the partial VP2 gene region of canine parvoviruses in domestic cats from Turkey.Virus genes · 2012Article
- Uterine adenocarcinoma with feline leukemia virus infection.Laboratory animal research · 2011Article
- Markers of feline leukaemia virus infection or exposure in cats from a region of low seroprevalence.Journal of feline medicine and surgery · 2011Article
- High prevalence of non-productive FeLV infection in necropsied cats and significant association with pathological findings.Veterinary immunology and immunopathology · 2010Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laboratory diagnosis of feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) usually involves both viruses, as the clinical signs are similar and coinfection may occur. Serological methods may not represent an accurate diagnosis: maternal antibodies or cross-reactions may give false positive results to FIV, and false negative results may occur in latent FeLV status, or in certain FIV infection stages. A nested polymerase chain reaction (PCR) technique was designed to detect FeLV, FIV and feline endogenous retrovirus simultaneously. The detection of endogenous sequences was considered indicative of successful DNA extraction. The technique was used to diagnose FIV and FeLV in the blood cells of 179 cats. The kappa value with the serological data was 0.69 for FeLV and 0.87 for FIV. The joint detection of FeLV and FIV by this novel nested PCR is sensitive, specific, fast and convenient, and its applicability for clinical diagnosis is promising, as the direct evidence of the presence of the virus is more realistic than the indirect data provided by the serological detection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.