ArticleJournal of biomolecular NMR2006
Solid-state NMR spectroscopy of 10% 13C labeled ubiquitin: spectral simplification and stereospecific assignment of isopropyl groups.
Article in Journal of biomolecular NMR, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- A random fractionalMagnetic resonance letters · 2026Article
- Cell-free synthesis of proteins with selectivelyMagnetic resonance (Gottingen, Germany) · 2023Article
- Temperature-Dependent Solid-State NMR Proton Chemical-Shift Values and Hydrogen Bonding.The journal of physical chemistry. B · 2021Article
- Spinning faster: protein NMR at MAS frequencies up to 126 kHz.Journal of biomolecular NMR · 2019Article
- Ubiquitin immobilized on mesoporous MCM41 silica surfaces - Analysis by solid-state NMR with biophysical and surface characterization.Biointerphases · 2017Article
- Partially-deuterated samples of HET-s(218-289) fibrils: assignment and deuterium isotope effect.Journal of biomolecular NMR · 2017Article
- An Efficient Labelling Approach to Harness Backbone and Side-Chain Protons in (1) H-Detected Solid-State NMR Spectroscopy.Angewandte Chemie (International ed. in English) · 2015Article
- Structural and functional implications of the QUA2 domain on RNA recognition by GLD-1.Nucleic acids research · 2014Article
- BSH-CP based 3D solid-state NMR experiments for protein resonance assignment.Journal of biomolecular NMR · 2014Article
- PAIN with and without PAR: variants for third-spin assisted heteronuclear polarization transfer.Journal of biomolecular NMR · 2013Article
- Secondary structural analysis of proteins based on (13)C chemical shift assignments in unresolved solid-state NMR spectra enhanced by fragmented structure database.Journal of biomolecular NMR · 2013Article
- 3D DUMAS: simultaneous acquisition of three-dimensional magic angle spinning solid-state NMR experiments of proteins.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2012Article
- Utilizing afterglow magnetization from cross-polarization magic-angle-spinning solid-state NMR spectroscopy to obtain simultaneous heteronuclear multidimensional spectra.The journal of physical chemistry. B · 2012Article
- Solid-state NMR spectroscopy of protein complexes.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Structural basis of pre-let-7 miRNA recognition by the zinc knuckles of pluripotency factor Lin28.Nature structural & molecular biology · 2011Article
- Three-dimensional deuterium-carbon correlation experiments for high-resolution solid-state MAS NMR spectroscopy of large proteins.Journal of biomolecular NMR · 2011Article
- The structure of human ubiquitin in 2-methyl-2,4-pentanediol: a new conformational switch.Protein science : a publication of the Protein Society · 2011Article
- Optimization of amino acid type-specific 13C and 15N labeling for the backbone assignment of membrane proteins by solution- and solid-state NMR with the UPLABEL algorithm.Journal of biomolecular NMR · 2011Article
- Assigning large proteins in the solid state: a MAS NMR resonance assignment strategy using selectively and extensively 13C-labelled proteins.Journal of biomolecular NMR · 2009Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the simplification of 13C-13C correlation spectra obtained from a microcrystalline protein sample expressed on a growth medium of 10% fully 13C labeled glucose diluted in 90% natural abundance glucose as compared to a fully labeled sample. Such a labeling scheme facilitates the backbone and side-chain resonance assignment of Phe, Tyr, His, Asp, Asn, Ile, Lys and Pro and yields an unambiguous stereospecific assignment of the valine Cgamma1, Cgamma2 13C resonances and of Leucine Cdelta2.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.