ArticleThe Journal of physiology2006
Molecular and electrophysiological characterization of transient receptor potential ion channels in the primary murine megakaryocyte.
Article in The Journal of physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- Pooled it
- Role of the mechanotransductor PIEZO1 in megakaryocyte differentiation.Journal of cellular and molecular medicine · 2024Article
- Article
- Mind the gap: connexins and pannexins in platelet function.Platelets · 2021Review
- Distribution and Assembly of TRP Ion Channels.Advances in experimental medicine and biology · 2021Article
- Mouse transient receptor potential channel type 6 selectively regulates agonist-induced platelet function.Biochemistry and biophysics reports · 2019Article
- Zinc Homeostasis in Platelet-Related Diseases.International journal of molecular sciences · 2019Review
- Modulation of MgThe journal of physiological sciences : JPS · 2019Article
- Pathophysiological Significance of Store-Operated Calcium Entry in Megakaryocyte Function: Opening New Paths for Understanding the Role of Calcium in Thrombopoiesis.International journal of molecular sciences · 2016Review
- Transcriptomic analysis of the ion channelome of human platelets and megakaryocytic cell lines.Thrombosis and haemostasis · 2016Article
- Defects in TRPM7 channel function deregulate thrombopoiesis through altered cellular Mg(2+) homeostasis and cytoskeletal architecture.Nature communications · 2016Article
- Increased expression of transient receptor potential canonical 6 (TRPC6) in differentiating human megakaryocytes.Cell biology international · 2016Article
- A critical role for the transient receptor potential channel type 6 in human platelet activation.PloS one · 2015Article
- TRPM2 contributes to antigen-stimulated Ca²⁺ influx in mucosal mast cells.Pflugers Archiv : European journal of physiology · 2013Article
- Redox signal-mediated sensitization of transient receptor potential melastatin 2 (TRPM2) to temperature affects macrophage functions.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Article
- Properties and therapeutic potential of transient receptor potential channels with putative roles in adversity: focus on TRPC5, TRPM2 and TRPA1.Current drug targets · 2011Review
- TRPM2 channel membrane currents in primary rat megakaryocytes were activated by the agonist ADP-ribose but not oxidative stress.The Journal of membrane biology · 2011Article
- TRPM2: a multifunctional ion channel for calcium signalling.The Journal of physiology · 2011Review
- Compensatory thrombopoietin production from the liver and bone marrow stimulates thrombopoiesis of living rat megakaryocytes in chronic renal failure.Nephron extra · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The molecular identity of platelet Ca(2+) entry pathways is controversial. Furthermore, the extent to which Ca(2+)-permeable ion channels are functional in these tiny, anucleate cells is difficult to assess by direct electrophysiological measurements. Recent work has highlighted how the primary megakaryocyte represents a bona fide surrogate for studies of platelet signalling, including patch clamp recordings of ionic conductances. We have now screened for all known members of the transient receptor potential (TRP) family of non-selective cation channels in murine megakaryocytes following individual selection of these rare marrow cells using glass micropipettes. RT-PCR detected messages for TRPC6 and TRPC1, which have been reported in platelets and megakaryocytic cell lines, and TRPM1, TRPM2 and TRPM7, which to date have not been demonstrated in cells of megakaryocytic/platelet lineage. Electrophysiological recordings demonstrated the presence of functional TRPM7, a constitutively active cation channel sensitive to intracellular Mg(2+), and TRPM2, an ADP-ribose-dependent cation channel activated by oxidative stress. In addition, the electrophysiological and pharmacological properties of the non-selective cation channels stimulated by the physiological agonist ADP are consistent with a major role for TRPC6 in this G-protein-coupled receptor-dependent Ca(2+) influx pathway. This study defines for the first time the principal TRP channels within the primary megakaryocyte, which represent candidates for Ca(2+) influx pathways activated by a diverse range of stimuli in the platelet and megakaryocyte.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.