ArticleThe Journal of physiology2006
Pattern of Ca2+ increase determines the type of secretory mechanism activated in dog pancreatic duct epithelial cells.
Article in The Journal of physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Signaling through CaCold Spring Harbor perspectives in biology · 2020Review
- Ion Channel Signature in Healthy Pancreas and Pancreatic Ductal Adenocarcinoma.Frontiers in pharmacology · 2020Review
- Molecular mechanisms of caffeine-mediated intestinal epithelial ion transports.British journal of pharmacology · 2019Article
- Minimizing ATP depletion by oxygen scavengers for single-molecule fluorescence imaging in live cells.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- KPflugers Archiv : European journal of physiology · 2016Article
- Contributions of protein kinases and β-arrestin to termination of protease-activated receptor 2 signaling.The Journal of general physiology · 2016Article
- Potassium channels in pancreatic duct epithelial cells: their role, function and pathophysiological relevance.Pflugers Archiv : European journal of physiology · 2015Review
- Epithelial monolayer culture system for real-time single-cell analyses.Physiological reports · 2014Article
- Acid-base transport in pancreas-new challenges.Frontiers in physiology · 2013Review
- The cystic fibrosis of exocrine pancreas.Cold Spring Harbor perspectives in medicine · 2013Review
- Actin cytoskeleton controls movement of intracellular organelles in pancreatic duct epithelial cells.Cell calcium · 2012Article
- Cyclic AMP potentiates Ca2+-dependent exocytosis in pancreatic duct epithelial cells.The Journal of general physiology · 2010Article
- Control of granule mobility and exocytosis by Ca2+ -dependent formation of F-actin in pancreatic duct epithelial cells.Traffic (Copenhagen, Denmark) · 2009Article
- Purinergic receptors in the endocrine and exocrine pancreas.Purinergic signalling · 2008Article
- Adenosine receptors in rat and human pancreatic ducts stimulate chloride transport.Pflugers Archiv : European journal of physiology · 2008Article
- Molecular basis of potassium channels in pancreatic duct epithelial cells.Channels (Austin, Tex.)Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Intracellular calcium concentration ([Ca(2+)](i)) is a key factor controlling secretion from various cell types. We investigated how different patterns of [Ca(2+)](i) signals evoke salt secretion via ion transport mechanisms and mucin secretion via exocytosis in dog pancreatic duct epithelial cells (PDEC). Activation of epithelial P2Y(2) receptors by UTP generated two patterns of [Ca(2+)](i) change: 2-10 microm UTP induced [Ca(2+)](i) oscillations, whereas 100 microm UTP induced a sustained [Ca(2+)](i) increase, both in the micromolar range. As monitored by carbon-fibre amperometry, the sustained [Ca(2+)](i) increase stimulated a larger increase in exocytosis than [Ca(2+)](i) oscillations, despite their similar amplitude. In contrast, patch-clamp recordings revealed that [Ca(2+)](i) oscillations synchronously activated a K(+) current as efficiently as the sustained [Ca(2+)](i) increase. This K(+) current was mediated by intermediate-conductance Ca(2+)-activated K(+) channels (32 pS at -100 mV) which were sensitive to charybdotoxin and resistant to TEA. Activation of these Ca(2+)-dependent K(+) channels hyperpolarized the plasma membrane from a resting potential of -40 mV to -90 mV, as monitored in perforated whole-cell configuration, in turn enhancing Na(+)-independent, Cl(-)-dependent and DIDS-sensitive HCO(3)(-) secretion, as monitored through changes in intracellular pH. PDEC therefore encode concentrations of purinergic agonists as different patterns of [Ca(2+)](i) changes, which differentially stimulate K(+) channels, the Cl(-)-HCO(3)(-) exchanger, and exocytosis. Thus, in addition to amplitude, the temporal pattern of [Ca(2+)](i) increases is an important mechanism for transducing extracellular stimuli into different physiological effects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.