ArticleGene therapy2006
Magnetic resonance imaging of viral particle biodistribution in vivo.
Article in Gene therapy, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 56 citations in OpenAlex.
- PET imaging of AAV9 and AAVBR1 trafficking in normal mice.Scientific reports · 2025Article
- Toxicological Aspects of Iron Oxide Nanoparticles.Advances in experimental medicine and biology · 2022Article
- Brome mosaic virus-like particles as siRNA nanocarriers for biomedical purposes.Beilstein journal of nanotechnology · 2020Article
- Comprehensive cytotoxicity studies of superparamagnetic iron oxide nanoparticles.Biochemistry and biophysics reports · 2018Review
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- Endocytic uptake pathways utilized by CPMV nanoparticles.Molecular pharmaceutics · 2013Article
- Hybrid nanoparticles for detection and treatment of cancer.Advanced materials (Deerfield Beach, Fla.) · 2012Review
- Brain microvascular endothelial cell association and distribution of a 5 nm ceria engineered nanomaterial.International journal of nanomedicine · 2012Article
- The application of nanoparticles in gene therapy and magnetic resonance imaging.Microscopy research and technique · 2011Review
- Superparamagnetic iron oxide nanoparticles: promises for diagnosis and treatment of cancer.International journal of molecular epidemiology and genetics · 2011Article
- Polymeric PARACEST MRI contrast agents as potential reporters for gene therapy.Organic & biomolecular chemistry · 2010Article
- Enabling individualized therapy through nanotechnology.Pharmacological research · 2010Review
- In vivo fluorescence imaging is well-suited for the monitoring of adenovirus directed transgene expression in living organisms.Molecular imaging and biology · 2010Article
- (Strept)avidin-displaying lentiviruses as versatile tools for targeting and dual imaging of gene delivery.Gene therapy · 2009Article
- Viral nanoparticles associate with regions of inflammation and blood brain barrier disruption during CNS infection.Journal of neuroimmunology · 2009Article
- Non-invasive genetic imaging for molecular and cell therapies of cancer.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2007Review
- Engineering targeted viral vectors for gene therapy.Nature reviews. Genetics · 2007Review
- SPECT/CT imaging of baculovirus biodistribution in rat.Gene therapy · 2007Article
- Baculovirus as a gene delivery vector: recent understandings of molecular alterations in transduced cells and latest applications.Biotechnology advancesReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe here a technique for the visualization of viral vector delivery by magnetic resonance imaging (MRI) in vivo. By conjugating avidin-coated baculoviral vectors (Baavi) with biotinylated ultra-small superparamagnetic iron oxide particles (USPIO), we are able to produce vector-related MRI contrast in the choroid plexus cells of rat brain in vivo over a period of 14 days. Ten microlitres of 2.5 x 10(10) PFU/ml nuclear-targeted LacZ-encoding Baavi with bUSPIO coating was injected into rat brain ventricles and visualized by MRI at 4.7 T. As baculoviruses exhibit restricted cell-type specificity in the rat brain, altered MRI contrast was detected in the choroid plexus of the injected ventricles. No specific signal loss was detected when wild-type baculoviruses or intact biotinylated USPIO particles were injected into the lateral ventricles. Cryosectioned brains were stained for nuclear-targeted beta-galactosidase gene expression, which was found to colocalize with MRI contrast. This study provides the first proof of principle for robust and non-invasive viral vector MRI by using avidin-displaying viruses in vivo. Considering the widespread use of MRI in current medical imaging, the approach is likely to provide numerous future applications in imaging of therapeutic gene transfer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.