ArticleNucleic acids research2006
The WD40-repeat protein Pwp1p associates in vivo with 25S ribosomal chromatin in a histone H4 tail-dependent manner.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 13 citations in OpenAlex.
- PWP1 transcriptionally regulates p53, modulating apoptosis and cell cycle to promote gastric cancer progression.Apoptosis : an international journal on programmed cell death · 2025Article
- PWP1 is overexpressed in hepatocellular carcinoma and facilitates liver cancer cell proliferation.Heliyon · 2024Article
- Systematic characterization of chromodomain proteins reveals an H3K9me1/2 reader regulating aging in C. elegans.Nature communications · 2023Article
- Maize Shrek1 encodes a WD40 protein that regulates pre-rRNA processing in ribosome biogenesis.The Plant cell · 2022Article
- Interaction of G-protein βγ complex with chromatin modulates GPCR-dependent gene regulation.PloS one · 2013Article
- Rrp5p, Noc1p and Noc2p form a protein module which is part of early large ribosomal subunit precursors in S. cerevisiae.Nucleic acids research · 2013Article
- Characterization of a highly conserved histone related protein, Ydl156w, and its functional associations using quantitative proteomic analyses.Molecular & cellular proteomics : MCP · 2012Article
- no child left behind encodes a novel chromatin factor required for germline stem cell maintenance in males but not females.Development (Cambridge, England) · 2011Article
- Antagonistic Gcn5-Hda1 interactions revealed by mutations to the Anaphase Promoting Complex in yeast.Cell division · 2011Article
- Identification and characterization of endonuclein binding proteins: evidence of modulatory effects on signal transduction and chaperone activity.BMC biochemistry · 2009Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The tails of core histones (H2A, H2B, H3 and H4) are critical for the regulation of chromatin dynamics. Each core histone tail is specifically recognized by various tail binding proteins. Here we screened for budding yeast histone H4-tail binding proteins in a protein differential display approach by two-dimensional gel electrophoresis (2DGE). To obtain highly enriched chromatin proteins, we used a Mg2+-dependent chromatin oligomerization technique. The Mg2+-dependent oligomerized chromatin from H4-tail deleted cells was compared with that from wild-type cells. We used mass spectrometry to identify 22 candidate proteins whose amounts were reduced in the oligomerized chromatin from the H4-tail deleted cells. A Saccharomyces Genome Database search revealed 10 protein complexes, each of which contained more than two candidate proteins. Interestingly, 7 out of the 10 complexes have the potential to associate with the H4-tail. We obtained in vivo evidence, by a chromatin immunoprecipitation assay, that one of the candidate proteins, Pwp1p, associates with the 25S ribosomal DNA (rDNA) chromatin in an H4-tail-dependent manner. We propose that the complex containing Pwp1p regulates the transcription of rDNA. Our results demonstrate that the protein differential display approach by 2DGE, using a histone-tail mutant, is a powerful method to identify histone-tail binding proteins.
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