ArticleJournal of bacteriology2006
Enhanced biofilm formation and loss of capsule synthesis: deletion of a putative glycosyltransferase in Porphyromonas gingivalis.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 120 citations in OpenAlex.
- Article
- Article
- A pleiotropic role of sialidase in the pathogenicity ofInfection and immunity · 2024Article
- Comparative analysis ofMicrobiology spectrum · 2024Article
- A comprehensive comparison of biofilm formation and capsule production for bacterial survival on hospital surfaces.Biofilm · 2023Article
- Periodontal Pathogens and Their Links to Neuroinflammation and Neurodegeneration.Microorganisms · 2023Review
- Growth ofJournal of oral microbiology · 2023Article
- Atypical cyclic di-AMP signaling is essential for Porphyromonas gingivalis growth and regulation of cell envelope homeostasis and virulence.NPJ biofilms and microbiomes · 2022Article
- Adaptation and phenotypic diversification of Bacillus thuringiensis biofilm are accompanied by fuzzy spreader morphotypes.NPJ biofilms and microbiomes · 2022Article
- D-arginine Enhances the Effect of Alpha-Amylase on DisassemblingFrontiers in bioengineering and biotechnology · 2022Article
- Anti-biofilm activities of coumarin as quorum sensing inhibitor forJournal of oral microbiology · 2022Article
- The human oral virome: Shedding light on the dark matter.Periodontology 2000 · 2021Review
- A PorX/PorY and σmSphere · 2021Article
- The dental plaque biofilm matrix.Periodontology 2000 · 2021Review
- AFrontiers in oral health · 2021Article
- A Novel Regulation of K-antigen Capsule Synthesis inFrontiers in oral health · 2021Article
- A novel gingipain regulatory gene in Porphyromonas gingivalis mediates host cell detachment and inhibition of wound closure.MicrobiologyOpen · 2020Article
- Diversity of mucoid to non-mucoid switch among carbapenemase-producing Klebsiella pneumoniae.BMC microbiology · 2020Article
- Diversity and evolution of surface polysaccharide synthesis loci in Enterobacteriales.The ISME journal · 2020Article
- Characterization ofMicrobiology (Reading, England) · 2019Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors.
Funding
Abstract
Periodontitis is a biofilm-mediated disease. Porphyromonas gingivalis is an obligate anaerobe consistently associated with severe manifestations of this disease. As an opportunistic pathogen, the ability to proliferate within and disseminate from subgingival biofilm (plaque) is central to its virulence. Here, we report the isolation of a P. gingivalis transposon insertion mutant altered in biofilm development and the reconstruction and characterization of this mutation in three different wild-type strains. The mutation responsible for the altered biofilm phenotype was in a gene with high sequence similarity ( approximately 61%) to a glycosyltransferase gene. The gene is located in a region of the chromosome that includes up to 16 genes predicted to be involved in the synthesis and transport of capsular polysaccharide. The phenotype of the reconstructed mutation in all three wild-type backgrounds is that of enhanced biofilm formation. In addition, in strain W83, a strain that is encapsulated, the glycosyltransferase mutation resulted in a loss of capsule. Further experiments showed that the W83 mutant strain was more hydrophobic and exhibited increased auto-aggregation. Our results indicate that we have identified a gene involved in capsular-polysaccharide synthesis in P. gingivalis and that the production of capsule prevented attachment and the initiation of in vitro biofilm formation on polystyrene microtiter plates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.