ArticleJournal of bacteriology2006
Transcription of the pst operon of Clostridium acetobutylicum is dependent on phosphate concentration and pH.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 54 citations in OpenAlex.
- Article
- Multiple clades of regulators contribute to bacterial phosphate homeostasis and pathogenesis.bioRxiv : the preprint server for biology · 2025Article
- Mathematical modelling of clostridial acetone-butanol-ethanol fermentation.Applied microbiology and biotechnology · 2017Review
- Elucidation of the roles of adhE1 and adhE2 in the primary metabolism of Clostridium acetobutylicum by combining in-frame gene deletion and a quantitative system-scale approach.Biotechnology for biofuels · 2016Article
- Changes in protein abundance are observed in bacterial isolates from a natural host.Frontiers in cellular and infection microbiology · 2015Article
- Global transcriptome analysis of Clostridium thermocellum ATCC 27405 during growth on dilute acid pretreated Populus and switchgrass.Biotechnology for biofuels · 2013Article
- Integrative modelling of pH-dependent enzyme activity and transcriptomic regulation of the acetone-butanol-ethanol fermentation of Clostridium acetobutylicum in continuous culture.Microbial biotechnology · 2013Article
- Parallel Evolution and Horizontal Gene Transfer of the pst Operon in Firmicutes from Oligotrophic Environments.International journal of evolutionary biology · 2011Article
- A systems biology approach to investigate the effect of pH-induced gene regulation on solvent production by Clostridium acetobutylicum in continuous culture.BMC systems biology · 2011Article
- A proteomic and transcriptional view of acidogenic and solventogenic steady-state cells of Clostridium acetobutylicum in a chemostat culture.Applied microbiology and biotechnology · 2010Article
- The role of PerR in O2-affected gene expression of Clostridium acetobutylicum.Journal of bacteriology · 2009Article
- The two-component system PhoPR of Clostridium acetobutylicum is involved in phosphate-dependent gene regulation.Journal of bacteriology · 2008Article
- Genetic and proteomic analyses of a proteasome-activating nucleotidase A mutant of the haloarchaeon Haloferax volcanii.Journal of bacteriology · 2008Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pst operon of Clostridium acetobutylicum ATCC 824 comprises five genes, pstS, pstC, pstA, pstB, and phoU, and shows a gene architecture identical to that of Escherichia coli. Deduced proteins are predicted to represent a high-affinity phosphate-specific ABC (ATP-binding cassette) transport system (Pst) and a protein homologous to PhoU, a negative phosphate regulon regulator. We analyzed the expression patterns of the pst operon in P(i)-limited chemostat cultures during acid production at pH 5.8 or solvent production at pH 4.5 and in response to P(i) pulses. Specific mRNA transcripts were found only when external P(i) concentrations had dropped below 0.2 mM. Two specific transcripts were detected, a 4.7-kb polycistronic mRNA spanning the whole operon and a quantitatively dominating 1.2-kb mRNA representing the first gene, pstS. The mRNA levels clearly differed depending on the external pH. The amounts of the full-length mRNA detected were about two times higher at pH 5.8 than at pH 4.5. The level of pstS mRNA increased by a factor of at least 8 at pH 5.8 compared to pH 4.5 results. Primer extension experiments revealed only one putative transcription start point 80 nucleotides upstream of pstS. Thus, additional regulatory sites are proposed in the promoter region, integrating two different extracellular signals, namely, depletion of inorganic phosphate and the pH of the environment. After phosphate pulses were applied to a phosphate-limited chemostat we observed faster phosphate consumption at pH 5.8 than at pH 4.5, although higher optical densities were recorded at pH 4.5.
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