ArticleJournal of bacteriology2006
DNA supercoiling and the Lrp protein determine the directionality of fim switch DNA inversion in Escherichia coli K-12.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- RstAB activates type 1 fimbriae to promote uropathogeniciScience · 2026Article
- Article
- Two-component system GrpP/GrpQ promotes pathogenicity of uropathogenic Escherichia coli CFT073 by upregulating type 1 fimbria.Nature communications · 2025Article
- Type 1 fimbrial phase variation in multidrug-resistant asymptomatic uropathogenic Escherichia coli clinical isolates upon adherence to HTB-4 cells.Folia microbiologica · 2024Article
- The DNA relaxation-dependent OFF-to-ON biasing of the type 1 fimbrial genetic switch requires the Fis nucleoid-associated protein.Microbiology (Reading, England) · 2023Article
- The Fis Nucleoid Protein Negatively Regulates the Phase VariationFrontiers in microbiology · 2022Article
- The leucine-responsive regulatory proteins/feast-famine regulatory proteins: an ancient and complex class of transcriptional regulators in bacteria and archaea.Critical reviews in biochemistry and molecular biology · 2021Review
- Flexible Metabolism and Suppression of Latent Enzymes Are Important forJournal of bacteriology · 2019Article
- DNA supercoiling and transcription in bacteria: a two-way street.BMC molecular and cell biology · 2019Review
- Article
- Article
- Phase variation of Clostridium difficile virulence factors.Gut microbes · 2018Review
- The interplay between DNA topology and accessory factors in site-specific recombination in bacteria and their bacteriophages.Science progress · 2016Review
- The regulatory role of DNA supercoiling in nucleoprotein complex assembly and genetic activity.Biophysical reviews · 2016Review
- Article
- Thermal control of virulence factors in bacteria: a hot topic.Virulence · 2014Review
- The type 1 pili regulator gene fimX and pathogenicity island PAI-X as molecular markers of uropathogenic Escherichia coli.Microbiology (Reading, England) · 2013Article
- Regulation ofWorld journal of clinical infectious diseases · 2011Article
- Sensitive and accurate identification of protein-DNA binding events in ChIP-chip assays using higher order derivative analysis.Nucleic acids research · 2011Article
- Role of decreased levels of Fis histone-like protein in Crohn's disease-associated adherent invasive Escherichia coli LF82 bacteria interacting with intestinal epithelial cells.Journal of bacteriology · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Site-specific recombinases of the integrase family usually require cofactors to impart directionality in the recombination reactions that they catalyze. The FimB integrase inverts the Escherichia coli fim switch (fimS) in the on-to-off and off-to-on directions with approximately equal efficiency. Inhibiting DNA gyrase with novobiocin caused inversion to become biased in the off-to-on direction. This directionality was not due to differential DNA topological distortion of fimS in the on and off phases by the activity of its resident P(fimA) promoter. Instead, the leucine-responsive regulatory (Lrp) protein was found to determine switching outcomes. Knocking out the lrp gene or abolishing Lrp binding sites 1 and 2 within fimS completely reversed the response of the switch to DNA relaxation. Inactivation of either Lrp site alone resulted in mild on-to-off bias, showing that they act together to influence the response of the switch to changes in DNA supercoiling. Thus, Lrp is not merely an architectural element organizing the fim invertasome, it collaborates with DNA supercoiling to determine the directionality of the DNA inversion event.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.