ArticleCellular immunology2006
DNase II deficiency impairs innate immune function in Drosophila.
Article in Cellular immunology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
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- Cytosolic mtDNA and associated EYA-mediated pro-inflammatory signaling modulate healthspan in Drosophila.Research square · 2025Article
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- Caspase inhibition improves viability and efficiency of liposomal transfection.Scientific reports · 2023Article
- Secreted mammalian DNases protect against systemic bacterial infection by digesting biofilms.The Journal of experimental medicine · 2023Article
- Updated roles of cGAS-STING signaling in autoimmune diseases.Frontiers in immunology · 2023Review
- A virus changed my life.Molecular biology of the cell · 2022Article
- Article
- Conserved and Distinct Elements of Phagocytosis in Human andInternational journal of molecular sciences · 2021Review
- Structure of acid deoxyribonuclease.Nucleic acids research · 2017Article
- Suppression of systemic autoimmunity by the innate immune adaptor STING.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A novel pathway of cell death in response to cytosolic DNA in Drosophila cells.Journal of innate immunity · 2015Article
- Persephone/Spätzle pathogen sensors mediate the activation of Toll receptor signaling in response to endogenous danger signals in apoptosis-deficient Drosophila.The Journal of biological chemistry · 2014Article
- Article
- The roles and acting mechanism of Caenorhabditis elegans DNase II genes in apoptotic dna degradation and development.PloS one · 2009Article
- Cell-autonomous requirement for DNaseII in nonapoptotic cell death.Cell death and differentiation · 2009Article
- The phylogeny and evolution of deoxyribonuclease II: an enzyme essential for lysosomal DNA degradation.Molecular phylogenetics and evolution · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
DNase II enzymes are highly conserved proteins that are required for the degradation of DNA within phagolysosomes. Engulfment of apoptotic cells and/or bacteria by phagocytic cells requires the function of DNase II to completely destroy ingested DNA. Mutation of the dnase II gene results in an increase of undegraded apoptotic DNA within phagocytic cells in mice and nematodes. Additionally, reduction of DNase II enzymatic activity in Drosophila melanogaster has been shown to lead to increased accumulation of DNA in the ovaries. Due to the importance of DNA clearance during infection, we hypothesized that a severe reduction of DNase II activity would result in diminished immune function and viability. To test this hypothesis, we knocked down DNase II activity in flies using RNAi. As expected, expression of a dnase II-RNAi construct in flies resulted in a dramatic reduction of DNase II activity and a significant decrease in total hemocyte numbers. Furthermore, infection of dnase II-RNAi flies with Gram negative or positive bacteria resulted in a severe reduction in fly viability. These results confirm that DNase II and the ability to clear macromolecular DNA is essential for maintaining proper immune function in Drosophila.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.