ArticleThe journal of physical chemistry. B2005
Metal-enhanced fluoroimmunoassay on a silver film by vapor deposition.
Article in The journal of physical chemistry. B, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 98 citations in OpenAlex.
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- Fabrication of Highly Packed Plasmonic Nanolens Array Using Polymer Nanoimprinted Nanodots for an Enhanced Fluorescence Substrate.Polymers · 2018Article
- Recent Advances in Biosensing With Photonic Crystal Surfaces: A Review.IEEE sensors journal · 2016Article
- Plasma-Induced Wafer-Scale Self-Assembly of Silver Nanoparticles and Application to Biochemical Sensing.Materials (Basel, Switzerland) · 2015Article
- Fluorescence quenching N,N-bis(2,6-dimethylphenyl)-3,4:9,10-perylenetetracarboxylic diimide (BDPD) laser dye by colloidal silver nanoparticles.Journal of fluorescence · 2015Article
- Article
- A straightforward immunoassay applicable to a wide range of antibodies based on surface enhanced fluorescence.Journal of fluorescence · 2013Article
- Plasmonic hotspots of dynamically assembled nanoparticles in nanocapillaries: Towards a micro ribonucleic acid profiling platform.Biomicrofluidics · 2013Article
- Immunoassays based on surface-enhanced fluorescence using gap-plasmon-tunable Ag bilayer nanoparticle films.Journal of fluorescence · 2013Article
- Plasmon-controlled fluorescence and single DNA strand sequenching.Proceedings of SPIE--the International Society for Optical Engineering · 2012Article
- Photonic crystal enhanced fluorescence for early breast cancer biomarker detection.Journal of biophotonics · 2012Review
- Plasmonic substrates for multiplexed protein microarrays with femtomolar sensitivity and broad dynamic range.Nature communications · 2011Article
- Metal-Enhanced Fluorescence from Nanoparticulate Zinc Films.The journal of physical chemistry. C, Nanomaterials and interfaces · 2008Article
- Enhanced fluorescence images for labeled cells on silver island films.Langmuir : the ACS journal of surfaces and colloids · 2008Article
- Metal Enhanced Fluorescence on Silicon Wafer Substrates.Chemical physics letters · 2008Article
- Single-molecule spectroscopic study of enhanced intrinsic phycoerythrin fluorescence on silver nanostructured surfaces.Analytical chemistry · 2008Article
- Plasmon-Coupled Fluorescence Probes: Effect of Emission Wavelength on Fluorophore-Labeled Silver Particles.The journal of physical chemistry. C, Nanomaterials and interfaces · 2008Article
- Single-cell fluorescence imaging using metal plasmon-coupled probe 2: single-molecule counting on lifetime image.Nano letters · 2008Article
- Single molecule photophysics near metallic nanostructures.Proceedings of SPIE--the International Society for Optical Engineering · 2008Article
- Single-Molecule Studies on Fluorescently Labeled Silver Particles: Effects of Particle Size.The journal of physical chemistry. C, Nanomaterials and interfaces · 2007Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We studied a fluoroimmunoassay using metal-enhanced fluorescence (MEF) detection on silver film generated by vapor deposition method. The morphology of the silver film was controlled through the thickness of the film. A silica layer was coated on the silver film to protect the film and separate the fluorophore from the metal surface. Rabbit immunoglobulin G (IgG) was adsorbed on the silica by physiosorption and then dye-labeled anti-rabbit IgG was bound to the immobilized rabbit IgG. It was observed that the fluorophore was quenched on a thin silver film (2 nm), enhanced on a thick film (>5 nm), and reached saturation (ca. 10 times enhancement) at 20 nm. The MEF was also dependent on the thickness of the silica with a maximum at 10 nm. The lowest lifetime was observed on the 20 nm silver film, which was consistent with the saturation of MEF. These results showed the properties of a silver film needed for a maximum increase of fluorescence intensity in a fluoroimmunoassay. Dependence of the MEF on the emission wavelength was also studied using different dye-labeled anti-rabbit IgGs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.