ArticleIn vitro cellular & developmental biology. Animal
Isolation and characterization of residual undifferentiated mouse embryonic stem cells from embryoid body cultures by fluorescence tracking.
Article in In vitro cellular & developmental biology. Animal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 43 citations in OpenAlex.
- Hyaluronan-Based Grafting Strategies for Liver Stem Cell Therapy and Tracking Methods.Stem cells international · 2019Review
- Progress and challenges in macroencapsulation approaches for type 1 diabetes (T1D) treatment: Cells, biomaterials, and devices.Biotechnology and bioengineering · 2016Review
- Differentiation of human skin-derived precursor cells into functional islet-like insulin-producing cell clusters.In vitro cellular & developmental biology. Animal · 2015Article
- Generating cartilage repair from pluripotent stem cells.Tissue engineering. Part B, Reviews · 2014Review
- Evidence for self-maintaining pluripotent murine stem cells in embryoid bodies.Stem cell reviews and reports · 2014Article
- Cell labeling and tracking method without distorted signals by phagocytosis of macrophages.Theranostics · 2014Article
- Transient Downregulation of Nanog and Oct4 Induced by DETA/NO Exposure in Mouse Embryonic Stem Cells Leads to Mesodermal/Endodermal Lineage Differentiation.Stem cells international · 2014Article
- Engineering of the embryonic and adult stem cell niches.Iranian Red Crescent medical journal · 2013Article
- Loss of Oct4 expression during the development of murine embryoid bodies.Developmental biology · 2012Article
- The next (re)generation of ovarian biology and fertility in women: is current science tomorrow's practice?Fertility and sterility · 2012Review
- Selective removal of undifferentiated embryonic stem cells from differentiation cultures through HSV1 thymidine kinase and ganciclovir treatment.Stem cell reviews and reports · 2010Article
- Excision of reprogramming transgenes improves the differentiation potential of iPS cells generated with a single excisable vector.Stem cells (Dayton, Ohio) · 2010Article
- Article
- Instructing an embryonic stem cell-derived oocyte fate: lessons from endogenous oogenesis.Endocrine reviews · 2009Review
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The differentiation of mouse embryonic stem (ES) cells can be induced in vitro after leukemia inhibitory factor (LIF) withdrawal and further enhanced by the formation of "embryoid body" (EB) aggregates. This strategy is being used in order to optimize differentiation protocols that would result in functional cells for experimental cell replacement therapies. However, this study presents the possibility for residual undifferentiated cells to survive after standard in vitro procedures. Mouse ES cells were stably transfected with the enhanced green fluorescent protein (EGFP), under the control of the Oct4 promoter, a transcription factor that is expressed in undifferentiated ES cells but down-regulated on differentiation. Residual fluorescent cells were isolated from EBs that were cultured in standard conditions in absence of LIF. These residual cells displayed recurrent gain of chromosomes 8 and 9. Residual fluorescent cells, further expanded in absence of LIF and cultured as EBs, still displayed a significant Oct4 expression in comparison with parental transfected ES cells. Consequently, these residual cells have an intrinsic resistance to differentiate. The behavior of these cells, observed in vitro, can be overcome in vivo, as they were able to induce teratomas in subcutaneously injected nude mice. Residual undifferentiated cells displayed slight levels of VASA and DAZL expression. These results demonstrate that mouse ES cells cultured in vitro, in standard conditions, can spontaneously acquire recurrent karyotypical changes that may promote an undifferentiated stage, being selected in standard culture conditions in vitro.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.