ArticleJournal of the American Chemical Society2006
Determining the topology of integral membrane peptides using EPR spectroscopy.
Article in Journal of the American Chemical Society, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 71 citations in OpenAlex.
- The Expression, Purification, Spectroscopic Characterization, and Membrane Topology Classification of KCNE4 from RecombinantThe journal of physical chemistry. B · 2025Article
- One Stone Four Birds: A Novel Liposomal Delivery System Multi-functionalized with Ginsenoside Rh2 for Tumor Targeting Therapy.Nano-micro letters · 2020Article
- Electron Paramagnetic Resonance as a Tool for Studying Membrane Proteins.Biomolecules · 2020Review
- Review
- Site-Directed Spin Labeling EPR for Studying Membrane Proteins.BioMed research international · 2018Review
- Probing topology and dynamics of the second transmembrane domain (M2δ) of the acetyl choline receptor using magnetically aligned lipid bilayers (bicelles) and EPR spectroscopy.Chemistry and physics of lipids · 2017Article
- Membrane Disruption Mechanism of a Prion Peptide (106-126) Investigated by Atomic Force Microscopy, Raman and Electron Paramagnetic Resonance Spectroscopy.The journal of physical chemistry. B · 2017Article
- Pulse EPR Measurements of Intramolecular Distances in a TOPP-Labeled Transmembrane Peptide in Lipids.Biophysical journal · 2016Article
- The rational search for selective anticancer derivatives of the peptide Trichogin GA IV: a multi-technique biophysical approach.Scientific reports · 2016Article
- A bifunctional spin label reports the structural topology of phospholamban in magnetically-aligned bicelles.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2016Article
- Optimization of bicelle lipid composition and temperature for EPR spectroscopy of aligned membranes.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2015Article
- CW dipolar broadening EPR spectroscopy and mechanically aligned bilayers used to measure distance and relative orientation between two TOAC spin labels on an antimicrobial peptide.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2014Article
- Peptide-lipid interactions: experiments and applications.International journal of molecular sciences · 2013Article
- Use of electron paramagnetic resonance to solve biochemical problems.Biochemistry · 2013Review
- When detergent meets bilayer: birth and coming of age of lipid bicelles.Progress in nuclear magnetic resonance spectroscopy · 2013Review
- The magic of bicelles lights up membrane protein structure.Chemical reviews · 2012Review
- Determining α-helical and β-sheet secondary structures via pulsed electron spin resonance spectroscopy.Biochemistry · 2012Article
- Enhancement of electron spin echo envelope modulation spectroscopic methods to investigate the secondary structure of membrane proteins.The journal of physical chemistry. B · 2012Article
- Determining the orientation and localization of membrane-bound peptides.Current protein & peptide science · 2012Review
- Distance measurements on a dual-labeled TOAC AChR M2δ peptide in mechanically aligned DMPC bilayers via dipolar broadening CW-EPR spectroscopy.The journal of physical chemistry. B · 2012Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
This paper reports on the development of a new structural biology technique for determining the membrane topology of an integral membrane protein inserted into magnetically aligned phospholipid bilayers (bicelles) using EPR spectroscopy. The nitroxide spin probe, 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (TOAC), was attached to the pore-lining transmembrane domain (M2delta) of the nicotinic acetylcholine receptor (AChR) and incorporated into a bicelle. The corresponding EPR spectra revealed hyperfine splittings that were highly dependent on the macroscopic orientation of the bicelles with respect to the static magnetic field. The helical tilt of the peptide can be easily calculated using the hyperfine splittings gleaned from the orientational dependent EPR spectra. A helical tilt of 14 degrees was calculated for the M2delta peptide with respect to the bilayer normal of the membrane, which agrees well with previous 15N solid-state NMR studies. The helical tilt of the peptide was verified by simulating the corresponding EPR spectra using the standardized MOMD approach. This new method is advantageous because: (1) bicelle samples are easy to prepare, (2) the helical tilt can be directly calculated from the orientational-dependent hyperfine splitting in the EPR spectra, and (3) EPR spectroscopy is approximately 1000-fold more sensitive than 15N solid-state NMR spectroscopy; thus, the helical tilt of an integral membrane peptide can be determined with only 100 microg of peptide. The helical tilt can be determined more accurately by placing TOAC spin labels at several positions with this technique.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.