ArticleCellular & molecular biology letters2006
Direct Rho-associated kinase inhibition [correction of inhibiton] induces cofilin dephosphorylation and neurite outgrowth in PC-12 cells.
Article in Cellular & molecular biology letters, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 52 citations in OpenAlex.
- Characterization of Lysine Methylation During Neuronal Differentiation of LUHMES cells.bioRxiv : the preprint server for biology · 2026Article
- Integrating machine learning and multi-omics analysis to reveal nucleotide metabolism-related immune genes and their functional validation in ischemic stroke.Frontiers in immunology · 2025Article
- Expression of E-cadherin by CD8Nature communications · 2024Article
- The RhoA-ROCK1/ROCK2 Pathway Exacerbates Inflammatory Signaling in Immortalized and Primary Microglia.Cells · 2023Article
- Advantages of Rho-associated kinases and their inhibitor fasudil for the treatment of neurodegenerative diseases.Neural regeneration research · 2022Review
- 4D imaging analysis of the aging mouse neural stem cell niche reveals a dramatic loss of progenitor cell dynamism regulated by the RHO-ROCK pathway.Stem cell reports · 2022Article
- Amyloid-β Induces Cdh1-Mediated Rock2 Stabilization Causing Neurodegeneration.Frontiers in pharmacology · 2022Article
- Advances in the Signaling Pathways Downstream of Glial-Scar Axon Growth Inhibitors.Frontiers in cellular neuroscience · 2020Review
- Comparative Study of ROCK1 and ROCK2 in Hippocampal Spine Formation and Synaptic Function.Neuroscience bulletin · 2019Article
- The Prenylflavonoid ENDF1 Overrules Central Nervous System Growth Inhibitors and Facilitates Regeneration of DRG Neurons.Frontiers in cellular neuroscience · 2019Article
- Calsenilin, a Presenilin Interactor, Regulates RhoA Signaling and Neurite Outgrowth.International journal of molecular sciences · 2018Article
- Topical administration of a Rock/Net inhibitor promotes retinal ganglion cell survival and axon regeneration after optic nerve injury.Experimental eye research · 2017Article
- MK-STYX Alters the Morphology of Primary Neurons, and Outgrowths in MK-STYX Overexpressing PC-12 Cells Develop a Neuronal Phenotype.Frontiers in molecular biosciences · 2017Article
- Two PTP receptors mediate CSPG inhibition by convergent and divergent signaling pathways in neurons.Scientific reports · 2016Article
- HIV-1 Tat Regulates Occludin and Aβ Transfer Receptor Expression in Brain Endothelial Cells via Rho/ROCK Signaling Pathway.Oxidative medicine and cellular longevity · 2016Article
- Annexin A2-dependent actin bundling promotes secretory granule docking to the plasma membrane and exocytosis.The Journal of cell biology · 2015Article
- Stem cells in neuroinjury and neurodegenerative disorders: challenges and future neurotherapeutic prospects.Neural regeneration research · 2014Review
- Article
- Inhibition of rho kinase enhances survival of dopaminergic neurons and attenuates axonal loss in a mouse model of Parkinson's disease.Brain : a journal of neurology · 2012Article
- Phospholipase Cdelta3 regulates RhoA/Rho kinase signaling and neurite outgrowth.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Axons fail to regenerate in the adult central nervous system (CNS) following injury. Developing strategies to promote axonal regeneration is therapeutically attractive for various CNS pathologies such as traumatic brain injury, stroke and Alzheimer's disease. Because the RhoA pathway is involved in neurite outgrowth, Rho-associated kinases (ROCKs), downstream effectors of GTP-bound Rho, are potentially important targets for axonal repair strategies in CNS injuries. We investigated the effects and downstream mechanisms of ROCK inhibition in promoting neurite outgrowth in a PC-12 cell model. Robust neurite outgrowth (NOG) was induced by ROCK inhibitors Y-27632 and H-1152 in a time-and dose-dependent manner. Dramatic cytoskeletal reorganization was noticed upon ROCK inhibition. NOG initiated within 5 to 30 minutes followed by neurite extension between 6 and 10 hours. Neurite processes were then sustained for over 24 hours. Rapid cofilin dephosphorylation was observed within 5 minutes of Y-27632 and H-1152 treatment. Re-phosphorylation was observed by 6 hours after Y-27632 treatment, while H-1152 treatment produced sustained cofilin dephosphorylation for over 24 hours. The results suggest that ROCK-mediated dephosphorylation of cofilin plays a role in the initiation of NOG in PC-12 cells.
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