ArticleCellular & molecular biology letters2006
The bacterial artificial chromosome (BAC) library of the narrow-leafed lupin (Lupinus angustifolius L.).
Article in Cellular & molecular biology letters, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- First draft genome and genetic diversity analysis of the economically important and vulnerable tree species Saraca asoca (Roxb.) W.J. de Wilde.Journal of genetics · 2026Article
- A pan-genome and chromosome-length reference genome of narrow-leafed lupin (Lupinus angustifolius) reveals genomic diversity and insights into key industry and biological traits.The Plant journal : for cell and molecular biology · 2022Article
- Progress of Genomics-Driven Approaches for Sustaining Underutilized Legume Crops in the Post-Genomic Era.Frontiers in genetics · 2022Review
- The Puzzling Fate of a Lupin Chromosome Revealed by Reciprocal Oligo-FISH and BAC-FISH Mapping.Genes · 2020Article
- A Tale of Two Families: Whole Genome and Segmental Duplications Underlie Glutamine Synthetase and Phosphoenolpyruvate Carboxylase Diversity in Narrow-Leafed Lupin (International journal of molecular sciences · 2020Article
- Validation of Diaporthe toxica resistance markers in European Lupinus angustifolius germplasm and identification of novel resistance donors for marker-assisted selection.Journal of applied genetics · 2020Article
- Photoperiod and Vernalization Control of Flowering-Related Genes: A Case Study of the Narrow-Leafed Lupin (Frontiers in plant science · 2020Article
- Candidate Domestication-Related Genes Revealed by Expression Quantitative Trait Loci Mapping of Narrow-Leafed Lupin (International journal of molecular sciences · 2019Article
- Development of gene-based molecular markers tagging low alkaloid pauper locus in white lupin (Lupinus albus L.).Journal of applied genetics · 2019Article
- Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution.Genes · 2019Article
- Identification and profiling of narrow-leafed lupin (Lupinus angustifolius) microRNAs during seed development.BMC genomics · 2019Article
- Article
- A comprehensive draft genome sequence for lupin (Lupinus angustifolius), an emerging health food: insights into plant-microbe interactions and legume evolution.Plant biotechnology journal · 2017Article
- Expansion of the phosphatidylethanolamine binding protein family in legumes: a case study of Lupinus angustifolius L. FLOWERING LOCUS T homologs, LanFTc1 and LanFTc2.BMC genomics · 2016Article
- Integration of Lupinus angustifolius L. (narrow-leafed lupin) genome maps and comparative mapping within legumes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016Article
- A First Glimpse of Wild Lupin Karyotype Variation As Revealed by Comparative Cytogenetic Mapping.Frontiers in plant science · 2016Article
- Transcriptome sequencing of different narrow-leafed lupin tissue types provides a comprehensive uni-gene assembly and extensive gene-based molecular markers.Plant biotechnology journal · 2015Article
- Structure, expression profile and phylogenetic inference of chalcone isomerase-like genes from the narrow-leafed lupin (Lupinus angustifolius L.) genome.Frontiers in plant science · 2015Article
- New evidence of ancestral polyploidy in the Genistoid legume Lupinus angustifolius L. (narrow-leafed lupin).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014Article
- Comparative genomics of Lupinus angustifolius gene-rich regions: BAC library exploration, genetic mapping and cytogenetics.BMC genomics · 2013Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The narrow-leafed lupin possesses valuable traits for environment-friendly agriculture and for the production of unconventional agricultural products. Despite various genetic and environmental studies, the breeding of improved cultivars has been slow due to the limited knowledge of its genomic structure. Further advances in genomics require, among other things, the availability of a genomic DNA library with large inserts. We report here on the construction of the first DNA library cloned in a BAC (bacterial artificial chromosome) vector from diploid Lupinus angustifolius L. cv. Sonet. The high molecular weight DNA used for its preparation was isolated from interphase nuclei that were purified by flow cytometry. The library comprises 55,296 clones and is ordered in 144x384-well microtitre plates. With an average insert size of 100 kb, the library represents six haploid genome equivalents. Thanks to the purification of the nuclei by flow cytometry, contamination with chloroplast DNA and mitochondrial DNA was negligible. The availability of a BAC library opens avenues for the development of a physical contig map and positional gene cloning, as well as for the analysis of the plant's genome structure and evolution.
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