ArticleGenes & development2006
Function of the MAPK scaffold protein, Ste5, requires a cryptic PH domain.
Article in Genes & development, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed.
- Phosphoregulation of the novel hemi-arrestin MAPK scaffold Sms1 prevents untimely mating.Nature communications · 2026Article
- Conserved and rewired MAPK networks in human fungal pathogens.FEMS yeast research · 2026Review
- Article
- Yeast mating: Ceramide acyl chain length matters?Cell cycle (Georgetown, Tex.) · 2018Article
- Structural basis of autoregulatory scaffolding by apoptosis signal-regulating kinase 1.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A link between very long chain fatty acid elongation and mating-specific yeast cell cycle arrest.Cell cycle (Georgetown, Tex.) · 2017Article
- Heterotrimeric G Protein-coupled Receptor Signaling in Yeast Mating Pheromone Response.The Journal of biological chemistry · 2016Review
- Localized Surface Plasmon Resonance Biosensing: Current Challenges and Approaches.Sensors (Basel, Switzerland) · 2015Review
- Plasma membrane aminoglycerolipid flippase function is required for signaling competence in the yeast mating pheromone response pathway.Molecular biology of the cell · 2015Article
- Dynamic ubiquitination of the mitogen-activated protein kinase kinase (MAPKK) Ste7 determines mitogen-activated protein kinase (MAPK) specificity.The Journal of biological chemistry · 2013Article
- Mate and fuse: how yeast cells do it.Open biology · 2013Review
- Quantitative measurement of protein relocalization in live cells.Biophysical journal · 2013Article
- Structurally unique interaction of RBD-like and PH domains is crucial for yeast pheromone signaling.Molecular biology of the cell · 2013Article
- Membrane-protein binding measured with solution-phase plasmonic nanocube sensors.Nature methods · 2012Article
- Response to hyperosmotic stress.Genetics · 2012Review
- Conformational control of the Ste5 scaffold protein insulates against MAP kinase misactivation.Science (New York, N.Y.) · 2012Article
- The regulation of filamentous growth in yeast.Genetics · 2012Review
- Fungal mating pheromones: choreographing the dating game.Fungal genetics and biology : FG & B · 2011Review
- Scaffold proteins: hubs for controlling the flow of cellular information.Science (New York, N.Y.) · 2011Review
- Distribution and functions of sterols and sphingolipids.Cold Spring Harbor perspectives in biology · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Ste5, the prototypic mitogen-activated protein kinase (MAPK) scaffold protein, associates with plasma membrane-tethered Gbetagamma freed upon pheromone receptor occupancy, thereby initiating downstream signaling. We demonstrate that this interaction and membrane binding of an N-terminal amphipathic alpha-helix (PM motif) are not sufficient for Ste5 action. Rather, Ste5 contains a pleckstrin-homology (PH) domain (residues 388-518) that is essential for its membrane recruitment and function. Altering residues (R407S K411S) equivalent to those that mediate phosphoinositide binding in other PH domains abolishes Ste5 function. The isolated PH domain, but not a R407S K411S derivative, binds phosphoinositides in vitro. Ste5(R407S K411S) is expressed normally, retains Gbetagamma and Ste11 binding, and oligomerizes, yet is not recruited to the membrane in response to pheromone. Artificial membrane tethering of Ste5(R407S K411S) restores signaling. R407S K411S loss-of-function mutations abrogate the constitutive activity of gain-of-function Ste5 alleles, including one (P44L) that increases membrane affinity of the PM motif. Thus, the PH domain is essential for stable membrane recruitment of Ste5, and this association is critical for initiation of downstream signaling because it allows Ste5-bound Ste11 (MAPKKK) to be activated by membrane-bound Ste20 (MAPKKKK).
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.