ArticleMolecular and cellular biology2006
Association between SAP and FynT: Inducible SH3 domain-mediated interaction controlled by engagement of the SLAM receptor.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 37 citations in OpenAlex.
- Wiskott-Aldrich syndrome protein interacts and inhibits diacylglycerol kinase alpha promoting IL-2 induction.Frontiers in immunology · 2023Article
- SLAM Associated Protein Signaling in T Cells: Tilting the Balance Toward Autoimmunity.Frontiers in immunology · 2021Review
- The SLAM family receptors: Potential therapeutic targets for inflammatory and autoimmune diseases.Autoimmunity reviews · 2018Review
- Review
- The Immunomodulatory Functions of Diacylglycerol Kinase ζ.Frontiers in cell and developmental biology · 2016Review
- EAT-2, a SAP-like adaptor, controls NK cell activation through phospholipase Cγ, Ca++, and Erk, leading to granule polarization.The Journal of experimental medicine · 2014Article
- The receptor Ly108 functions as a SAP adaptor-dependent on-off switch for T cell help to B cells and NKT cell development.Immunity · 2012Article
- Characterization of Ly108 in the thymus: evidence for distinct properties of a novel form of Ly108.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Article
- SAP-mediated inhibition of diacylglycerol kinase α regulates TCR-induced diacylglycerol signaling.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Biochemical and genetic evidence for a SAP-PKC-theta interaction contributing to IL-4 regulation.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- SLAM family receptors and the SLAM-associated protein (SAP) modulate T cell functions.Seminars in immunopathology · 2010Review
- Itk tyrosine kinase substrate docking is mediated by a nonclassical SH2 domain surface of PLCgamma1.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Up on the tightrope: natural killer cell activation and inhibition.Nature immunology · 2008Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SAP is an intracellular adaptor molecule composed almost exclusively of an SH2 domain. It is mutated in patients with X-linked lymphoproliferative disease, a human immunodeficiency. Several immune abnormalities were also identified in SAP-deficient mice. By way of its SH2 domain, SAP interacts with tyrosine-based motifs in the cytoplasmic domain of SLAM family receptors. SAP promotes SLAM family receptor-induced protein tyrosine phosphorylation, due to its capacity to recruit the Src-related kinase FynT. This unusual property relies on the existence of a second binding surface in the SAP SH2 domain, centered on arginine 78 of SAP, that binds directly to the FynT SH3 domain. Herein, we wanted to further understand the mechanisms controlling the interaction between SLAM-SAP and FynT. Our experiments showed that, unlike conventional associations mediated by SH3 domains, the interaction of the FynT SH3 domain with SLAM-SAP was strictly inducible. It was absolutely dependent on engagement of SLAM by extracellular ligands. We obtained evidence that this inducibility was not due to increased binding of SLAM to SAP following SLAM engagement. Furthermore, it could occur independently of any appreciable SLAM-dependent biochemical signal. In fact, our data indicated that the induced association of the FynT SH3 domain with SLAM-SAP was triggered by a change in the conformation of SLAM-associated SAP caused by SLAM engagement. Together, these data elucidate further the events initiating SLAM-SAP signaling in immune cells. Moreover, they identify a strictly inducible interaction mediated by an SH3 domain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.