ArticleProceedings of the National Academy of Sciences of the United States of America2006
Phenotypic effects of membrane protein overexpression in Saccharomyces cerevisiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- Yeast Stress Response to Synthetic Constructs.ACS synthetic biology · 2026Review
- ML-enhanced peroxisome capacity enables compartmentalization of multienzyme pathway.Nature chemical biology · 2025Article
- Highly complete long-read genomes reveal pangenomic variation underlying yeast phenotypic diversity.Genome research · 2023Article
- A modular cloning (MoClo) toolkit for reliable intracellular protein targeting in the yeastMicrobial cell (Graz, Austria) · 2023Article
- Growth Inhibition by Amino Acids inMicroorganisms · 2020Article
- Expression of Leptospira membrane proteins Signal Peptidase (SP) and Leptospira Endostatin like A (Len A) in BL-21(DE3) is toxic to the host cells.Journal, genetic engineering & biotechnology · 2018Article
- Dynamically reshaping signaling networks to program cell fate via genetic controllers.Science (New York, N.Y.) · 2013Article
- Evaluating the fitness cost of protein expression in Saccharomyces cerevisiae.Genome biology and evolution · 2013Article
- Manipulating the genetic code for membrane protein production: what have we learnt so far?Biochimica et biophysica acta · 2012Review
- Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography.Protein expression and purification · 2010Article
- Tuning microbial hosts for membrane protein production.Microbial cell factories · 2009Review
- Selecting optimum eukaryotic integral membrane proteins for structure determination by rapid expression and solubilization screening.Journal of molecular biology · 2009Article
- Molecular code for protein insertion in the endoplasmic reticulum membrane is similar for N(in)-C(out) and N(out)-C(in) transmembrane helices.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Legionella eukaryotic-like type IV substrates interfere with organelle trafficking.PLoS pathogens · 2008Article
- Functional importance of individual rRNA 2'-O-ribose methylations revealed by high-resolution phenotyping.RNA (New York, N.Y.) · 2008Article
- High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Characterization of three classes of membrane proteins involved in fungal azole resistance by functional hyperexpression in Saccharomyces cerevisiae.Eukaryotic cell · 2007Article
- Characteristics affecting expression and solubilization of yeast membrane proteins.Journal of molecular biology · 2007Article
- PROPHECY--a yeast phenome database, update 2006.Nucleic acids research · 2007Article
- A global topology map of the Saccharomyces cerevisiae membrane proteome.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Large-scale protein overexpression phenotype screens provide an important complement to the more common gene knockout screens. Here, we have targeted the so far poorly understood Saccharomyces cerevisiae membrane proteome and report growth phenotypes for a strain collection overexpressing approximately 600 C-terminally tagged integral membrane proteins grown both under normal and three different stress conditions. Although overexpression of most membrane proteins reduce the growth rate in synthetic defined medium, we identify a large number of proteins that, when overexpressed, confer specific resistance to various stress conditions. Our data suggest that regulation of glycosylphosphatidylinositol anchor biosynthesis and the Na(+)/K(+) homeostasis system constitute major downstream targets of the yeast PKA/RAS pathway and point to a possible connection between the early secretory pathway and the cells' response to oxidative stress. We also have quantified the expression levels for >550 membrane proteins, facilitating the choice of well expressing proteins for future functional and structural studies.
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