ArticleReproductive biology and endocrinology : RB&E2006
FSH regulates acetycholine production by ovarian granulosa cells.
Article in Reproductive biology and endocrinology : RB&E, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Beyond apoptosis: evidence of other regulated cell death pathways in the ovary throughout development and life.Human reproduction update · 2023Pooled it
- The investigation of cholinergic receptor muscarinic 1 activity in the rat ovary with induced ovarian hyperstimulation.Turkish journal of obstetrics and gynecology · 2023Article
- The role of the autonomic nervous system in polycystic ovary syndrome.Frontiers in endocrinology · 2023Review
- Muscarinic receptors in the rat ovary are involved in follicular development but not in steroid secretion.Physiological reports · 2022Article
- Participation of the Cholinergic System in the Development of Polycystic Ovary Syndrome.Molecules (Basel, Switzerland) · 2021Article
- The NADPH oxidase 4 is a major source of hydrogen peroxide in human granulosa-lutein and granulosa tumor cells.Scientific reports · 2019Article
- Neuropeptide and steroid hormone mediators of neuroendocrine regulation.Journal of neuroendocrinology · 2018Review
- Acetylcholine and necroptosis are players in follicular development in primates.Scientific reports · 2018Article
- L-DOPA in the hu man ovarian follicular fluid acts as an antioxidant factor on granulosa cells.Journal of ovarian research · 2016Article
- In vivo blockade of acetylcholinesterase increases intraovarian acetylcholine and enhances follicular development and fertility in the rat.Scientific reports · 2016Article
- Pro-nerve growth factor in the ovary and human granulosa cells.Hormone molecular biology and clinical investigation · 2015Article
- Ovulation requires the activation on proestrus of M₁ muscarinic receptors in the left ovary.Endocrine · 2015Article
- Regulator of G-protein signaling 2 (RGS2) suppresses premature calcium release in mouse eggs.Development (Cambridge, England) · 2015Article
- Readthrough acetylcholinesterase (AChE-R) and regulated necrosis: pharmacological targets for the regulation of ovarian functions?Cell death & disease · 2015Article
- Expression of the beta-2 adrenergic receptor (ADRB-2) in human and monkey ovarian follicles: a marker of growing follicles?Journal of ovarian research · 2015Article
- Decorin is a part of the ovarian extracellular matrix in primates and may act as a signaling molecule.Human reproduction (Oxford, England) · 2012Article
- Oxytocin receptors in the primate ovary: molecular identity and link to apoptosis in human granulosa cells.Human reproduction (Oxford, England) · 2010Article
- Dopamine receptor repertoire of human granulosa cells.Reproductive biology and endocrinology : RB&E · 2007Article
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundIt has been previously shown that cultured granulosa cells (GCs) derived from human ovarian preovulatory follicles contain choline acetyltransferase (ChAT), the enzyme responsible for acetylcholine (ACh) synthesis. They also produce ACh and express functional muscarinic ACh receptors. ACh can act on GCs to increase proliferation, disrupt gap junctional communication, alter intracellular calcium levels, as well as expression of transcription factors, suggesting an unrecognized role of ACh in GC function. To gain further insights into the possible role of ACh in the ovary, we examined ChAT expression in the gland before and after birth, as well as in adults, and studied the regulation of ACh production by FSH.
methodsChAT immunohistochemistry was performed using ovarian samples of different species and ages (embryonic, postnatal and adult rats and mice, including embryonic ovaries from mice null for ChAT, neonatal and adult rhesus monkeys and adult humans). ACh was measured by HPLC and/or a fluorescence based method in rat ovaries and in a FSH receptor-expressing cell line (rat GFSHR-17) cultured with or without FSH.
resultsIn adult rat, as well as in all other species, ovarian ChAT immunoreactivity is associated with GCs of antral follicles, but not with other structures, indicating that GCs are the only ovarian source of ACh. Indeed ACh was clearly detected in adult rat ovaries by two methods. ChAT immunoreactivity is absent from embryonic and/or neonatal ovaries (mouse/rat and monkey) and ovarian development in embryonic mice null for ChAT appears normal, suggesting that ACh is not involved in ovarian or follicular formation. Since ChAT immunoreactivity is present in GCs of large follicles and since the degree of the ChAT immunoreactivity increases as antral follicles grow, we tested whether ACh production is stimulated by FSH. Rat GFSHR-17 cells that stably express the FSH receptor, respond to FSH with an increase in ACh production.
conclusionACh and ChAT are present in GCs of growing follicles and FSH, the major driving force of follicular growth, stimulates ACh production. Since ACh stimulates proliferation and differentiation processes in cultured GCs, we suggest that ACh may act in the growing ovarian follicle as a local mediator of some of the actions ascribed to FSH.
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