ArticleBiochemistry2006
Efficient substrate cleavage catalyzed by hammerhead ribozymes derivatized with selenium for X-ray crystallography.
Article in Biochemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Data.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Synthesis and optical behaviors of 6-seleno-deoxyguanosine.Science China. Chemistry · 2014Article
- Synthesis of novel di-Se-containing thymidine and Se-DNAs for structure and function studies.Science China. Chemistry · 2013Article
- Synthesis of the tellurium-derivatized phosphoramidites and their incorporation into DNA oligonucleotides.Current protocols in nucleic acid chemistry · 2011Article
- Facile synthesis of nucleoside 5'-(α-P-seleno)-triphosphates and phosphoroselenoate RNA transcription.RNA (New York, N.Y.) · 2011Article
- Protection-free one-pot synthesis of 2'-deoxynucleoside 5'-triphosphates and DNA polymerization.Organic letters · 2011Article
- Key labeling technologies to tackle sizeable problems in RNA structural biology.International journal of molecular sciences · 2008Article
- Selenium derivatization of nucleic acids for phase and structure determination in nucleic acid X-ray crystallography.International journal of molecular sciences · 2008Article
- Coupling of fast and slow modes in the reaction pathway of the minimal hammerhead ribozyme cleavage.Biophysical journal · 2007Article
- A general strategy to solve the phase problem in RNA crystallography.Structure (London, England : 1993) · 2007Article
- Synthesis of a 2'-Se-thymidine phosphoramidite and its incorporation into oligonucleotides for crystal structure study.Organic letters · 2007Article
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3 authors at 2 institutions in 1 country.
Funding
Abstract
Because oxygen and selenium are in the same group (Family VI) in the periodic table, the site-specific mutagenesis at the atomic level by replacing RNA oxygen with selenium can provide insights on the structure and function of catalytic RNAs. We report here the first Se-derivatized ribozymes transcribed with all nucleoside 5'-(alpha-P-seleno)triphosphates (NTPalphaSe, including A, C, G, and U). We found that T7 RNA polymerase recognizes NTPalphaSe Sp diastereomers as well as the natural NTPs, whereas NTPalphaSe Rp diastereomers are neither substrates nor inhibitors. We also demonstrated the catalytic activity of these Se-derivatized hammerhead ribozymes by cleaving the RNA substrate, and we found that these phosphoroselenoate ribozymes can be as active as the native one. These hammerhead ribozymes site-specifically mutagenized by selenium reveal the close relationship between the catalytic activities and the replaced oxygen atoms, which provides insight on the participation of oxygen in catalysis or intramolecular interaction. This demonstrates a convenient strategy for the mechanistic study of functional RNAs. In addition, the active ribozymes site-specifically derivatized by selenium will allow for convenient MAD phasing in X-ray crystal structure studies.
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