ArticleProceedings of the National Academy of Sciences of the United States of America2006
Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
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Who cites it
110 citing papers in PubMed, 357 citations in OpenAlex.
- Structural and biochemical basis for activity of Aspergillus nidulans α-1,3-glucanases from glycoside hydrolase family 71.Communications biology · 2025Article
- An l-fucose-responsive transcription factor cross-regulates the expression of a diverse array of carbohydrate-active enzymes in Trichoderma reesei.PLoS genetics · 2025Article
- Characterization of glycoside hydrolases involved in xyloglucan degradation in the thermophilic bacterium Thermotoga maritima.Scientific reports · 2025Article
- Developmental landscape and asymmetric gene expression in the leaf vasculature ofHorticulture research · 2025Article
- Biochemical Characterization and Polyester-Binding/Degrading Capability of Two Cutinases fromMicroorganisms · 2025Article
- Identification and structural characterization of a novel acetyl xylan esterase from Aspergillus oryzae.The FEBS journal · 2025Article
- Modification of xylan in secondary walls alters cell wall biosynthesis and wood formation programs and improves saccharification.Plant biotechnology journal · 2025Article
- Integrative dynamics of cell wall architecture and plant growth under salt stress.Frontiers in plant science · 2025Review
- Transcriptome Profiling, Cloning, and Characterization of AnGlu04478, a Ginsenoside Hydrolyzing β-Glucosidase from Aspergillus niger NG1306.Current microbiology · 2024Article
- Multiomics dissection of Brassica napus L. lateral roots and endophytes interactions under phosphorus starvation.Nature communications · 2024Article
- Integrity of xylan backbone affects plant responses to drought.Frontiers in plant science · 2024Article
- Cloning, Expression, Purification, and Characterization of a Novel β-Galactosidase/α-L-Arabinopyranosidase fromMolecules (Basel, Switzerland) · 2023Article
- Structure and function characterization of the α-L-arabinofuranosidase from the white-rot fungus Trametes hirsuta.Applied microbiology and biotechnology · 2023Article
- Structure and function of microbial α-l-fucosidases: a mini review.Essays in biochemistry · 2023Review
- Fungal Glycoside Hydrolases Display Unique Specificities for Polysaccharides andMicroorganisms · 2023Article
- Identification of fungal lignocellulose-degrading biocatalysts secreted by Phanerochaete chrysosporium via activity-based protein profiling.Communications biology · 2022Article
- Integrated methylome and transcriptome analysis unravel the cold tolerance mechanism in winter rapeseed(Brassica napus L.).BMC plant biology · 2022Article
- Article
- Characterization of an extracellular α-xylosidase involved in xyloglucan degradation in Aspergillus oryzae.Applied microbiology and biotechnology · 2022Article
- Discovery of novel secretome CAZymes fromFrontiers in bioengineering and biotechnology · 2022Article
50 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombinant enzymes were active in enzyme assays, and optima for pH and temperature were established. A subset of the enzymes was used to fragment polysaccharides from the irregular xylem 9 (irx9) mutant of Arabidopsis. The analysis revealed a decrease in the abundance of xylan in the mutant, indicating that the IRX9 gene, which encodes a putative family 43 glycosyltransferase, is required for xylan synthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.