ArticleJournal of virology2006
A poxvirus host range protein, CP77, binds to a cellular protein, HMG20A, and regulates its dissociation from the vaccinia virus genome in CHO-K1 cells.
Article in Journal of virology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- Poxvirus Host-Range Determinants: SAMD9/9L and Beyond.Annual review of virology · 2025Review
- Cross-species transmission and host range genes in poxviruses.Virologica Sinica · 2024Review
- Review
- Identification and Biochemical Characterization of High Mobility Group Protein 20A as a Novel CaBiomolecules · 2021Article
- Construction and purification of ANK gene deleted recombinant goatpox virus.Virusdisease · 2020Article
- Genomic analysis reveals an exogenous viral symbiont with dual functionality in parasitoid wasps and their hosts.PLoS pathogens · 2020Article
- Identification of CP77 as the Third Orthopoxvirus SAMD9 and SAMD9L Inhibitor with Unique Specificity for a Rodent SAMD9L.Journal of virology · 2019Article
- Circular RNA ABCB10 promotes hepatocellular carcinoma progression by increasing HMG20A expression by sponging miR-670-3p.Cancer cell international · 2019Article
- Mutagenic repair of double-stranded DNA breaks in vaccinia virus genomes requires cellular DNA ligase IV activity in the cytosol.The Journal of general virology · 2018Article
- Evaluation of Viral Genome Copies Within Viral Factories on Different DNA Viruses.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2018Article
- Genetic Analysis of a Commercial Egg Laying Line Challenged With Newcastle Disease Virus.Frontiers in genetics · 2018Article
- Production of a Chikungunya Vaccine Using a CHO Cell and Attenuated Viral-Based Platform Technology.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Poxviral ankyrin proteins.Viruses · 2015Review
- EVM005: an ectromelia-encoded protein with dual roles in NF-κB inhibition and virulence.PLoS pathogens · 2014Article
- The Role of F-Box Proteins during Viral Infection.International journal of molecular sciences · 2013Article
- The poxvirus C7L host range factor superfamily.Current opinion in virology · 2012Review
- Article
- The interaction with HMG20a/b proteins suggests a potential role for beta-dystrobrevin in neuronal differentiation.The Journal of biological chemistry · 2010Article
- Addition of poly(A) and poly(A)-rich tails during RNA degradation in the cytoplasm of human cells.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Poxvirus proteomics and virus-host protein interactions.Microbiology and molecular biology reviews : MMBR · 2009Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vaccinia virus does not grow in Chinese hamster ovary (CHO-K1) cells in the absence of a viral host range factor, cowpox protein CP77. In this study, CP77 was fused to the C terminus of green fluorescence protein (GFP-CP77) and a series of nested deletion mutants of GFP-CP77 was constructed for insertion into a vaccinia virus host range mutant, VV-hr, and expressed from a viral early promoter. Deletion mapping analyses demonstrated that the N-terminal 352 amino acids of CP77 were sufficient to support vaccinia virus growth in CHO-K1 cells, whereas the C-terminal residues 353 to 668 were dispensable. In yeast two-hybrid analyses, CP77 bound to a cellular protein, HMG20A, and GST pulldown analyses showed that residues 1 to 234 of CP77 were sufficient for this interaction. After VV-hr virus infection of CHO-K1 cells, HMG20A was translocated from the nucleus to viral factories and bound to the viral genome via the HMG box region. In control VV-hr-infected CHO-K1 cells, binding of HMG20A to the viral genome persisted from 2 to 8 h postinfection (h p.i.); in contrast, when CP77 was expressed, the association of HMG20A with viral genome was transient, with little HMG20A remaining bound at 8 h p.i. This indicates that dissociation of HMG20A from viral factories correlates well with CP77 host range activity in CHO-K1 cells. Finally, in cells expressing a CP77 deletion protein (amino acids 277 to 668) or a DeltaANK5 mutant that did not support vaccinia virus growth and did not contain the HMG20A binding site, HMG20A remained bound to viral DNA, demonstrating that the binding of CP77 to HMG20A is essential for its host range function. In summary, our data revealed that a novel cellular protein, HMG20A, the dissociation of which from viral DNA is regulated by CP77, providing the first cellular target regulated by viral host range CP77 protein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.