ArticleMolecular biology of the cell2006
A global, myosin light chain kinase-dependent increase in myosin II contractility accompanies the metaphase-anaphase transition in sea urchin eggs.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- CaCurrent biology : CB · 2025Article
- Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The nanoscale organization of the Wnt signaling integrator Dishevelled in the vegetal cortex domain of an egg and early embryo.PloS one · 2021Article
- Rac and Arp2/3-Nucleated Actin Networks Antagonize Rho During Mitotic and Meiotic Cleavages.Frontiers in cell and developmental biology · 2020Article
- Biochemical alterations in the oocyte in support of early embryonic development.Cellular and molecular life sciences : CMLS · 2017Review
- Influence of cell polarity on early development of the sea urchin embryo.Developmental dynamics : an official publication of the American Association of Anatomists · 2015Article
- Supervillin binding to myosin II and synergism with anillin are required for cytokinesis.Molecular biology of the cell · 2013Article
- Centralspindlin and chromosomal passenger complex behavior during normal and Rappaport furrow specification in echinoderm embryos.Cytoskeleton (Hoboken, N.J.) · 2012Article
- Separation anxiety: stress, tension and cytokinesis.Experimental cell research · 2012Review
- Myosin light chain kinases and phosphatase in mitosis and cytokinesis.Archives of biochemistry and biophysics · 2011Review
- On the cutting edge: post-translational modifications in cytokinesis.Trends in cell biology · 2011Review
- Cortical mechanics and meiosis II completion in mammalian oocytes are mediated by myosin-II and Ezrin-Radixin-Moesin (ERM) proteins.Molecular biology of the cell · 2010Article
- Deficiency in myosin light-chain phosphorylation causes cytokinesis failure and multipolarity in cancer cells.Oncogene · 2010Article
- Novel interactors and a role for supervillin in early cytokinesis.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Making the cut: the chemical biology of cytokinesis.ACS chemical biology · 2010Review
- Multi-scale mechanics from molecules to morphogenesis.The international journal of biochemistry & cell biology · 2009Review
- Bisphenol A directly targets tubulin to disrupt spindle organization in embryonic and somatic cells.ACS chemical biology · 2008Article
- The roles of Ca2+, downstream protein kinases, and oscillatory signaling in regulating fertilization and the activation of development.Developmental biology · 2008Review
- Distinct pathways for the early recruitment of myosin II and actin to the cytokinetic furrow.Molecular biology of the cell · 2008Article
- Phospholipase C and myosin light chain kinase inhibition define a common step in actin regulation during cytokinesis.BMC cell biology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Myosin II is the force-generating motor for cytokinesis, and although it is accepted that myosin contractility is greatest at the cell equator, the temporal and spatial cues that direct equatorial contractility are not known. Dividing sea urchin eggs were placed under compression to study myosin II-based contractile dynamics, and cells manipulated in this manner underwent an abrupt, global increase in cortical contractility concomitant with the metaphase-anaphase transition, followed by a brief relaxation and the onset of furrowing. Prefurrow cortical contractility both preceded and was independent of astral microtubule elongation, suggesting that the initial activation of myosin II preceded cleavage plane specification. The initial rise in contractility required myosin light chain kinase but not Rho-kinase, but both signaling pathways were required for successful cytokinesis. Last, mobilization of intracellular calcium during metaphase induced a contractile response, suggesting that calcium transients may be partially responsible for the timing of this initial contractile event. Together, these findings suggest that myosin II-based contractility is initiated at the metaphase-anaphase transition by Ca2+-dependent myosin light chain kinase (MLCK) activity and is maintained through cytokinesis by both MLCK- and Rho-dependent signaling. Moreover, the signals that initiate myosin II contractility respond to specific cell cycle transitions independently of the microtubule-dependent cleavage stimulus.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.