ArticleEukaryotic cell2006
Synergy among differentially regulated repressors of the ribonucleotide diphosphate reductase genes of Saccharomyces cerevisiae.
Article in Eukaryotic cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Replication-IDentifier links epigenetic and metabolic pathways to the replication stress response.Nature communications · 2025Article
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- Setosphaeria turcica ATR turns off appressorium-mediated maize infection and triggers melanin-involved self-protection in response to genotoxic stress.Molecular plant pathology · 2020Article
- Increased heme synthesis in yeast induces a metabolic switch from fermentation to respiration even under conditions of glucose repression.The Journal of biological chemistry · 2017Article
- Leveraging DNA damage response signaling to identify yeast genes controlling genome stability.G3 (Bethesda, Md.) · 2015Article
- DNA Damage Response Checkpoint Activation Drives KP1019 Dependent Pre-Anaphase Cell Cycle Delay in S. cerevisiae.PloS one · 2015Article
- Activator and repressor functions of the Mot3 transcription factor in the osmostress response of Saccharomyces cerevisiae.Eukaryotic cell · 2013Article
- Phosphoproteomic analysis of protein kinase C signaling in Saccharomyces cerevisiae reveals Slt2 mitogen-activated protein kinase (MAPK)-dependent phosphorylation of eisosome core components.Molecular & cellular proteomics : MCP · 2013Article
- Sen1p contributes to genomic integrity by regulating expression of ribonucleotide reductase 1 (RNR1) in Saccharomyces cerevisiae.PloS one · 2013Article
- Ixr1 is required for the expression of the ribonucleotide reductase Rnr1 and maintenance of dNTP pools.PLoS genetics · 2011Article
- The stress response factors Yap6, Cin5, Phd1, and Skn7 direct targeting of the conserved co-repressor Tup1-Ssn6 in S. cerevisiae.PloS one · 2011Article
- The Human Scavenger Receptor CD36: glycosylation status and its role in trafficking and function.The Journal of biological chemistry · 2009Article
- A novel mechanism of antagonism between ATP-dependent chromatin remodeling complexes regulates RNR3 expression.Molecular and cellular biology · 2009Article
- Evidence for lesion bypass by yeast replicative DNA polymerases during DNA damage.Nucleic acids research · 2008Article
- Trm9-catalyzed tRNA modifications link translation to the DNA damage response.Molecular cell · 2007Article
- Multiple basic helix-loop-helix proteins regulate expression of the ENO1 gene of Saccharomyces cerevisiae.Eukaryotic cell · 2007Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Ssn6/Tup1 general repression complex represses transcription of a number of regulons through recruitment by regulon-specific DNA-binding repressors. Rox1 and Mot3 are Ssn6/Tup1-recruiting, DNA-binding proteins that repress the hypoxic genes, and Rfx1 is a Ssn6/Tup1-recruiting, a DNA-binding protein that represses the DNA damage-inducible genes. We previously reported that Rox1 and Mot3 functioned synergistically to repress a subset of the hypoxic genes and that this synergy resulted from an indirect interaction through Ssn6. We report here cross-regulation between Rox1 and Mot3 and Rfx1 in the regulation of the RNR genes encoding ribonucleotide diphosphate reductase. Using a set of strains containing single and multiple mutations in the repressor encoding genes and lacZ fusions to the RNR2 to -4 genes, we demonstrated that Rox1 repressed all three genes and that Mot3 repressed RNR3 and RNR4. Each repressor could act synergistically with the others, and synergy required closely spaced sites. Using artificial constructs containing two repressor sites, we confirmed that all three proteins could function synergistically but that two Rox1 sites or two Rfx1 sites could not. The significance of this synergy lies in the ability to repress gene transcription strongly under normal growth conditions, and yet allow robust induction under conditions that inactivate only one of the repressors. Since the interaction between the proteins is indirect, the evolution of dually regulated genes requires only the acquisition of closely spaced repressor sites.
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