ArticleThe Biochemical journal2006
Insulin induction of glucokinase and fatty acid synthase in hepatocytes: analysis of the roles of sterol-regulatory-element-binding protein-1c and liver X receptor.
Article in The Biochemical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- Targeting cancer metabolism: Therapeutic potential of the fatty acid synthase (FASN) inhibitors.Critical reviews in oncology/hematology · 2025Review
- Characterization of aniScience · 2023Article
- Alterations of Central Liver Metabolism of Pediatric Patients with Non-Alcoholic Fatty Liver Disease.International journal of molecular sciences · 2022Article
- Transcriptomic changes in western mosquitofish (Gambusia affinis) liver following benzo[a]pyrene exposure.Environmental science and pollution research international · 2020Article
- Molecular mechanism of the anti-diabetic activity of an identified oligosaccharide fromResearch in pharmaceutical sciences · 2020Article
- PAS Kinase deficiency alters the glucokinase function and hepatic metabolism.Scientific reports · 2018Article
- Glucagon and Insulin Cooperatively Stimulate Fibroblast Growth Factor 21 Gene Transcription by Increasing the Expression of Activating Transcription Factor 4.The Journal of biological chemistry · 2017Article
- Linoleic and α-linolenic fatty acid consumption over three generations exert cumulative regulation of hepatic expression of genes related to lipid metabolism.Genes & nutrition · 2014Article
- Sterol regulatory element-binding protein-1 (SREBP-1) is required to regulate glycogen synthesis and gluconeogenic gene expression in mouse liver.The Journal of biological chemistry · 2014Article
- Glucagon stimulates hepatic FGF21 secretion through a PKA- and EPAC-dependent posttranscriptional mechanism.PloS one · 2014Article
- Article
- Expression of the human glucokinase gene: important roles of the 5' flanking and intron 1 sequences.PloS one · 2012Article
- Chylomicron remnants and nonesterified fatty acids differ in their ability to inhibit genes involved in lipogenesis in rats.The Journal of nutrition · 2011Article
- Transcriptional regulation of glucose sensors in pancreatic β-cells and liver: an update.Sensors (Basel, Switzerland) · 2010Review
- Retinoids synergize with insulin to induce hepatic Gck expression.The Biochemical journal · 2009Article
- Fibroblast growth factor-19, a novel factor that inhibits hepatic fatty acid synthesis.The Journal of biological chemistry · 2009Article
- Down-regulation of hepatic HNF4alpha gene expression during hyperinsulinemia via SREBPs.Molecular endocrinology (Baltimore, Md.) · 2009Article
- Peroxisome proliferator-activated receptor-alpha modulates insulin gene transcription factors and inflammation in adipose tissues in mice.Molecular and cellular biochemistry · 2009Article
- Molecular physiology of mammalian glucokinase.Cellular and molecular life sciences : CMLS · 2009Review
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcription activator SREBP-1c (sterol-regulatory-element-binding protein-1c) is induced by insulin in the liver and is considered a master regulator of lipogenic genes such as FASN (fatty acid synthase). The question of whether SREBP-1c is also a mediator of insulin action on the regulatory enzyme of glucose metabolism GCK (glucokinase) is controversial. In the present paper, we induced SREBP-1c to various levels with insulin or the liver X receptor ligand T0901317 in primary hepatocytes and asked if these levels correlated with those of GCK or FASN mRNA expression, using the latter as positive control. Insulin and T0901317 triggered the accumulation of precursor and processed forms of SREBP-1c to similar levels and with comparable kinetics, and both effectors together caused synergistic increases in SREBP-1c protein levels. These effects were accompanied by commensurate elevation of FASN mRNA, notably by a synergistic response to both effectors. By contrast, GCK mRNA was unresponsive to T0901317 and was induced only by insulin. Treatment of hepatocytes with insulin and/or T0901317 resulted in the recruitment of SREBP-1c to the FASN promoter as shown by chromatin immunoprecipitation, whereas SREBP-1c did not bind to the GCK promoter. Lastly, we observed that the glycogen synthase kinase-3 inhibitor SB216763 produced a small increase in SREBP-1c protein level, which was further augmented in the presence of T0901317. The level of FASN mRNA varied in parallel with SREBP-1c, while GCK mRNA was unaffected. Collectively, these results showed that increases in SREBP-1c were neither necessary nor sufficient for GCK induction in hepatocytes, while at the same time they underscored the role of SREBP-1c as a key regulator of FASN.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.