ArticlePlant cell reports2006
Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules.
Article in Plant cell reports, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 57 citations in OpenAlex.
- Multi-omics analysis reveals the positive impact of differential chloroplast activity during in vitro regeneration of barley.Plant molecular biology · 2024Article
- Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing.International journal of molecular sciences · 2023Review
- A CRISPR/Cas9-Based System with Controllable Auto-Excision Feature Serving Cisgenic Plant Breeding and Beyond.International journal of molecular sciences · 2022Article
- Direct Germline Transformation of Cotton Meristem Explants With No Selection.Frontiers in plant science · 2020Article
- Cas Endonuclease Technology-A Quantum Leap in the Advancement of Barley and Wheat Genetic Engineering.International journal of molecular sciences · 2019Review
- Barley HvPAPhy_a as transgene provides high and stable phytase activities in mature barley straw and in grains.Plant biotechnology journal · 2017Article
- ROOT HAIR DEFECTIVE SIX-LIKE Class I Genes Promote Root Hair Development in the Grass Brachypodium distachyon.PLoS genetics · 2016Article
- Advances in Agrobacterium tumefaciens-mediated genetic transformation of graminaceous crops.Protoplasma · 2016Review
- Insights into the multifaceted application of microscopic techniques in plant tissue culture systems.Planta · 2015Review
- Optimization of multiple shoot induction and plant regeneration in Indian barley (Hordeum vulgare) cultivars using mature embryos.Saudi journal of biological sciences · 2013Article
- Less is more: strategies to remove marker genes from transgenic plants.BMC biotechnology · 2013Review
- The elimination of a selectable marker gene in the doubled haploid progeny of co-transformed barley plants.Plant molecular biology · 2013Article
- Genotypic differences in callus induction and plant regeneration from mature embryos of barley (Hordeum vulgare L.).Journal of Zhejiang University. Science. B · 2011Article
- UCE: A uracil excision (USER)-based toolbox for transformation of cereals.Plant methods · 2010Article
- Agrobacterium-mediated gene transfer to cereal crop plants: current protocols for barley, wheat, triticale, and maize.International journal of plant genomics · 2009Article
- Transformation of different barley (Hordeum vulgare L.) cultivars by Agrobacterium tumefaciens infection of in vitro cultured ovules.Plant cell reports · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report on a novel transformation procedure for barley by Agrobacterium infection of in vitro cultured ovules. Ovules of the cultivar Golden Promise were isolated a few hours after pollination and infected with the Agrobacterium tumefaciens strain AGL0 carrying the binary vector pVec8-GFP. The vector harboured a hygromycin resistance gene and the green fluorescence protein (GFP) gene. GFP-expressing embryos were isolated from the ovules, regenerated to plants and investigated by Southern blot analysis. Transformation frequencies amounted to 3.1% with hygromycin selection and 0.8% without selection. Mendelian inheritance and stable expression of the GFP gene was confirmed in 18 independent lines over two generations. We conclude that the described technique allows for the rapid and direct generation of high quality transgenic plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.