ArticleProceedings of the National Academy of Sciences of the United States of America2006
Mutant LYS2 mRNAs retained and degraded in the nucleus of Saccharomyces cerevisiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Nrd1p identifies aberrant and natural exosomal target messages during the nuclear mRNA surveillance in Saccharomyces cerevisiae.Nucleic acids research · 2021Article
- Nuclear mRNA degradation tunes the gain of the unfolded protein response in Saccharomyces cerevisiae.Nucleic acids research · 2018Article
- mRNA quality control pathways in Saccharomyces cerevisiae.Journal of biosciences · 2013Review
- Aptamer-based molecular imaging.Protein & cell · 2012Review
- The exozyme model: a continuum of functionally distinct complexes.RNA (New York, N.Y.) · 2011Review
- hNUDT16: a universal decapping enzyme for small nucleolar RNA and cytoplasmic mRNA.Protein & cell · 2011Article
- Frame-disrupting mutations elicit pre-mRNA accumulation independently of frame disruption.Nucleic acids research · 2010Article
- A systematic characterization of Cwc21, the yeast ortholog of the human spliceosomal protein SRm300.RNA (New York, N.Y.) · 2009Article
- Evolutionary conservation supports ancient origin for Nudt16, a nuclear-localized, RNA-binding, RNA-decapping enzyme.Nucleic acids research · 2008Article
- The exosome and RNA quality control in the nucleus.EMBO reports · 2007Review
- A novel plasmid-based microarray screen identifies suppressors of rrp6Delta in Saccharomyces cerevisiae.Molecular and cellular biology · 2007Article
- mRNA nuclear export and human disease.Disease models & mechanismsArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We previously demonstrated that mRNAs retained in the nucleus of Saccharomyces cerevisiae are subjected to a degradation system-designated DRN (degradation of mRNA in the nucleus), that is diminished in cbc1-Delta or cbc2-Delta mutants lacking components of the cap-binding complex and in rrp6-Delta mutants lacking Rrp6p, a 3' to 5' nuclear exonuclease. Two mutants, lys2-187 and lys2-121, were uncovered by screening numerous lys2 mutants for suppression by cbc1-Delta and rrp6-Delta. Both mutants were identical and contained the two base changes, one of which formed a TGA nonsense codon. LYS2 mRNAs from the lys2-187 and related mutants were rapidly degraded, and the degradation was suppressed by cbc1-Delta and rrp6-Delta. The U1A-GFP imaging procedure was used to show that the lys2-187 mRNA was partially retained in the nucleus, explaining the susceptibility to DRN. The creation of several derivatives of lys2-187 by site-directed mutagenesis revealed that the in-frame TGA by itself was not responsible for the increased susceptibility to DRN. Thus, mRNAs susceptible to DRN can be formed by a 2-bp change. Furthermore, this "retention signal" causing susceptibility to DRN is lost by altering one of the base pairs, establishing that mRNAs susceptible and unsusceptible to DRN can be attributed to a single nucleotide in the proper context.
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